Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients
| Autor: | Mette Kolpen, Kasper Nørskov Kragh, Peter Østrup Jensen, Oana Ciofu, Niels Høiby, Signe Agnete Møller, Thomas Bjarnsholt |
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| Rok vydání: | 2019 |
| Předmět: |
Pulmonary and Respiratory Medicine
Cystic Fibrosis Multidrug tolerance Neutrophils medicine.drug_class medicine.medical_treatment Antibiotics medicine.disease_cause Models Biological Cystic fibrosis Bacterial Adhesion Microbiology Tobramycin medicine Humans Lung Respiratory Tract Infections Pathogen Bacteriological Techniques Hyperbaric Oxygenation Pseudomonas aeruginosa business.industry medicine.disease Anti-Bacterial Agents Oxygen medicine.anatomical_structure Pediatrics Perinatology and Child Health business Adjuvant medicine.drug |
| Zdroj: | Journal of Cystic Fibrosis. 18:657-664 |
| ISSN: | 1569-1993 |
| DOI: | 10.1016/j.jcf.2019.01.005 |
| Popis: | Background: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Recent work has shown that re‑oxygenation of hypoxic aggregates by hyperbaric oxygen (O2) treatment (HBOT: 100% O2 at 2.8 bar) will increase killing of aggregating bacteria by antibiotics. This is relevant for treatment of infected CF patients where bacterial aggregates are found in the endobronchial secretions that are depleted of O2 by the metabolism of polymorphonuclear leukocytes (PMNs). The main objective of this study was to investigate the effect of HBOT as an adjuvant to tobramycin treatment of aggregates formed by P. aeruginosa isolates from CF patients. Methods: The effect was tested using a model with bacterial aggregates embedded in agarose. O2 profiling was used to confirm re‑oxygenation of aggregates. Results: We found that HBOT was able to significantly enhance the effect of tobramycin against aggregates of all the P. aeruginosa isolates in vitro. The effect was attributed to increased O2 levels leading to increased growth and thus increased uptake of and killing by tobramycin. Conclusions: Re‑oxygenation may in the future be a clinical possibility as adjuvant to enhance killing by antibiotics in cystic fibrosis lung infections. |
| Databáze: | OpenAIRE |
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