Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
| Autor: | Henry S. Marr, Meredith K. Sherrill, Leah A. Cohn, Adam J. Birkenheuer, Angela Trieu, Michael G. Levy, Jaime L. Tarigo, Chris Hung, Li Liang, Mason V. Reichard, Jennifer E. Thomas, Hilton Outi, David McK. Bird, Denise L. Doolan, Adam Vigil, Rie Nakajima, Megan E. Schreeg, Phillip L. Felgner, Elizabeth H. Scholl |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
and promotion of well-being Biochemistry & Molecular Biology Microarray 030231 tropical medicine Clinical Biochemistry Virulence lcsh:Medicine Protein microarray Vaccine Related 03 medical and health sciences 0302 clinical medicine Rare Diseases Antigen Genetics Molecular Biology Piroplasmid biology Felis Prevention lcsh:R General Medicine Cytauxzoonosis biology.organism_classification Prevention of disease and conditions Virology Cytauxzoon 030104 developmental biology Infectious Diseases Orphan Drug Good Health and Well Being Immunization 3.4 Vaccines Molecular Medicine Infection Expression library immunization Vaccine Cytauxzoon felis Biotechnology |
| Zdroj: | Clinical proteomics, vol 15, iss 1 Clinical Proteomics, Vol 15, Iss 1, Pp 1-17 (2018) |
| Popis: | Background Cytauxzoonosis is a disease of felids in North America caused by the tick-transmitted apicomplexan parasite Cytauxzoon felis. Cytauxzoonosis is particularly virulent for domestic cats, but no vaccine currently exists. The parasite cannot be cultivated in vitro, presenting a significant limitation for vaccine development. Methods Recent sequencing of the C. felis genome has identified over 4300 putative protein-encoding genes. From this pool we constructed a protein microarray containing 673 putative C. felis proteins. This microarray was probed with sera from C. felis-infected and naïve cats to identify differentially reactive antigens which were incorporated into two expression library vaccines, one polyvalent and one monovalent. We assessed the efficacy of these vaccines to prevent of infection and/or disease in a tick-challenge model. Results Probing of the protein microarray resulted in identification of 30 differentially reactive C. felis antigens that were incorporated into the two expression library vaccines. However, expression library immunization failed to prevent infection or disease in cats challenged with C. felis. Conclusions Protein microarray facilitated high-throughput identification of novel antigens, substantially increasing the pool of characterized C. felis antigens. These antigens should be considered for development of C. felis vaccines, diagnostics, and therapeutics. |
| Databáze: | OpenAIRE |
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