Optimized, Fast-Throughput UHPLC-DAD Based Method for Carotenoid Quantification in Spinach, Serum, Chylomicrons, and Feces
| Autor: | Eva Arrigoni, Lars O. Dragsted, Pia Lisbeth Madsen, Jane N. Eriksen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lutein Resolution (mass spectrometry) 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Feces Spinacia oleracea Chylomicrons Humans Carotenoid Chromatography High Pressure Liquid chemistry.chemical_classification 030109 nutrition & dietetics Chromatography biology 010401 analytical chemistry General Chemistry biology.organism_classification Carotenoids Lycopene 0104 chemical sciences chemistry Echinenone Spinach General Agricultural and Biological Sciences Chylomicron |
| Zdroj: | Journal of agricultural and food chemistry. 65(4) |
| ISSN: | 1520-5118 |
| Popis: | An improved UHPLC-DAD-based method was developed and validated for quantification of major carotenoids present in spinach, serum, chylomicrons, and feces. Separation was achieved with gradient elution within 12.5 min for six dietary carotenoids and the internal standard, echinenone. The proposed method provides, for all standard components, resolution1.1, linearity covering the target range (R0.99), LOQ0.035 mg/L, and intraday and interday RSDs2 and 10%, respectively. Suitability of the method was tested on biological matrices. Method precision (RSD%) for carotenoid quantification in serum, chylomicrons, and feces was below 10% for intra- and interday analysis, except for lycopene. Method accuracy was consistent with mean recoveries ranging from 78.8 to 96.9% and from 57.2 to 96.9% for all carotenoids, except for lycopene, in serum and feces, respectively. Additionally, an interlaboratory validation study on spinach at two institutions showed no significant differences in lutein or β-carotene content, when evaluated on four occasions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|