Long-term stability studies on protection against Newcastle disease by commercial live vaccine used in Brazil
| Autor: | Clarice Weis Arns, Fernando Rosado Spilki, Simone Alves Mendes Ribeiro, Maria da Glória Buzinaro, Margarida Maria Hoppner Zaroni, M.A Orsi, Luciano Doretto Júnior, S.C.A Camillo |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Veterinary medicine
Time Factors Newcastle Disease Bioengineering Applied Microbiology and Biotechnology Newcastle disease Specimen Handling Fresh air Drug Stability Medicine Animals Statistical analysis Pharmacology Attenuated vaccine General Immunology and Microbiology biology business.industry Viral Vaccine Significant difference Commerce Titrimetry Viral Vaccines General Medicine biology.organism_classification Virology Vaccination Cold Temperature Titer Treatment Outcome business Chickens Brazil Biotechnology |
| Zdroj: | Biologicals : journal of the International Association of Biological Standardization. 37(4) |
| ISSN: | 1095-8320 |
| Popis: | The thermostability (TS) and efficacy offered by live vaccines against Newcastle disease strains B1, La Sota, VG-GA and Ulster, produced or imported by four Brazilian laboratories, were evaluated during their validity period. Kinetic profiles were obtained from samples conserved in refrigerators during 0, 4, 8, 12, 16, 20 and 24 months after their manufacturing. The statistical analysis of the vaccine titre effect obtained by the fresh air (FA) method showed that the vaccine profiles were parallel and coincident, presenting a significant descending trend. The vaccine titres and efficiency proofs at the end of the validity period were above the level of legislation requirements and showed an average loss in titre of 0.40 and 0.66 log(10,) within the first and second validity years, respectively. The titre obtained by TS, within the month after manufacturing, had no significant difference from the titre obtained by FA within 24 months after manufacturing, being their pairs of observations positively correlated (r=0.49, p=0.0003), showing that the TS method, which anticipates the vaccines' performance at the end of the validity period, can substitute the FA method 24 months after manufacturing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect