Retinoic acid enhances IL-1 beta expression in myeloid leukemia cells and in human monocytes
| Autor: | Matikainen S, Serkkola E, Mikko Hurme |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Lipopolysaccharides
Sulfonamides Transcription Genetic Immunology Gene Expression Tretinoin Blotting Northern Isoquinolines Monocytes Piperazines Bucladesine Leukemia Myeloid 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Tumor Cells Cultured Humans Tetradecanoylphorbol Acetate Immunology and Allergy RNA Messenger Protein Kinase Inhibitors Interleukin-1 |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.147.1.162 |
| Popis: | We have examined the role of retinoic acid (RA), the biologically active metabolite of vitamin A, in expression of the IL-1 beta gene in the human myeloid leukemia cell line THP-1 and in human monocytes. Both protein kinase C-activating phorbol esters, e.g., PMA, and LPS induce IL-1 beta expression in these cells. Physiologic RA concentrations alone were not able to induce any IL-1 beta production, but they strongly enhanced the PMA-induced IL-1 beta protein production and mRNA accumulation in both human monocytes and in THP-1 cells. Nuclear run-off analysis revealed that the enhancing effect was at the transcriptional level. RA also slightly potentiated LPS-induced IL-1 beta expression in THP-1 cells but not in human monocytes. These data suggest that RA can be a strong up-regulator of IL-1 production, but its strength varies depending on the nature of the activating signal. |
| Databáze: | OpenAIRE |
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