In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres

Autor: Michael F. Haller, Patrice Laquerriere, Anthony Z. Faranesh, Monet T. Nastley, Elliot R. McVeigh, Kam W. Leong, Cristina Perez de la Cruz
Přispěvatelé: Laurent-Maquin, Dominique, Department of Biomedical Engineering [Baltimore] ( DBE ), Johns Hopkins University ( JHU ), Laboratory of Cardiac Energetics ( LCE ), National Heart-Lung, and Blood Institute-National Institutes of Health, Supramolecular Structure and Function Resource ( SSFR ), Office of Research Services-National Institutes of Health
Rok vydání: 2004
Předmět:
MESH : Cell Line
Gadolinium DTPA
Vascular Endothelial Growth Factor A
MESH : Polyglycolic Acid
Polymers
Gadolinium
Contrast Media
law.invention
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
law
[ SDV.IB ] Life Sciences [q-bio]/Bioengineering
chemistry.chemical_classification
Drug Carriers
MESH : Gadolinium DTPA
MESH : Cell Division
Infusion Pumps
Implantable
Polymer
Microspheres
Vascular endothelial growth factor
PLGA
Biodegradation
Environmental
MESH : Vascular Endothelial Growth Factor A
Drug delivery
MESH : Microspheres
Cell Division
MESH : Drug Carriers
MRI contrast agent
chemistry.chemical_element
Nanotechnology
In Vitro Techniques
Article
Cell Line
MESH : Biodegradation
Humans
Radiology
Nuclear Medicine and imaging
Lactic Acid
MESH : Polymers
[SDV.IB] Life Sciences [q-bio]/Bioengineering
MESH : Contrast Media
MESH : Cell Div
MESH : Humans
MESH : Infusion Pumps
Implantable
MESH : In Vitro
Biodegradable polymer
chemistry
MESH : Lactic Acid
Electron microscope
Polyglycolic Acid
Biomedical engineering
Zdroj: Magnetic Resonance in Medicine
Magnetic Resonance in Medicine, Wiley, 2004, 51, pp.1265-71. 〈10.1002/mrm.20092〉
ISSN: 1522-2594
0740-3194
DOI: 10.1002/mrm.20092
Popis: A drug delivery vehicle was constructed that could be visualized noninvasively with MRI. The biodegradable polymer poly(DL-lactic-co-glycolic acid) (PLGA) was used to fabricate microspheres containing vascular endothelial growth factor (VEGF) and the MRI contrast agent gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). The microspheres were characterized in terms of size, drug and contrast agent encapsulation, and degradation rate. The PLGA microspheres had a mean diameter of 48 +/- 18 microm. The gadolinium loading was 17 +/- 3 microg/mg polymer and the VEGF loading was 163 +/- 22 ng/mg polymer. Electron microscopy revealed that the Gd was dispersed throughout the microspheres and it was confirmed that the Gd loading was sufficient to visualize the microspheres under MRI. VEGF and Gd-DTPA were released from the microspheres in vitro over a period of approximately 6 weeks in three phases: a burst, followed by a slow steady-state, then a rapid steady-state. Biodegradable Gd-doped microspheres can be effectively used to deliver drugs in a sustained manner, while being monitored noninvasively with MRI.
Databáze: OpenAIRE
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