Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2
| Autor: | Lisa Shoff, Wei-wei Wang, Feng Wang, Feng Guo, Shuo Chen, Yuan Yang, Qingping Gao, Li-An Wu, Wentong Li, Hui-Hsiu Chuang |
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| Jazyk: | angličtina |
| Předmět: |
Cellular differentiation
Mesenchymal cell differentiation Bone Morphogenetic Protein 2 Biology Transfection Bone morphogenetic protein 2 Article Mesoderm Mice stomatognathic system Animals Humans Antigens Viral Tumor Dental papilla Dental Papilla SV-40 T antigen Cells Cultured Odontoblasts Mesenchymal stem cell Gene Expression Regulation Developmental Cell Differentiation Epithelial Cells General Medicine Anatomy Cell Biology Cell biology stomatognathic diseases Odontoblast Dental papilla mesenchymal cells Odontogenesis Stem cell Tooth Immortalised cell line Immortalization Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. Animal |
| ISSN: | 1071-2690 |
| DOI: | 10.1007/s11626-013-9641-1 |
| Popis: | Odontogenesis is the result of the reciprocal interactions between epithelial–mesenchymal cells leading to terminally differentiated odontoblasts. This process from dental papilla mesenchymal cells to odontoblasts is regulated by a complex signaling pathway. When isolated from the developing tooth germs, odontoblasts quickly lose their potential to maintain the odontoblast-specific phenotype. Therefore, generation of an odontoblast-like cell line would be a good surrogate model for studying the dental mesenchymal cell differentiation into odontoblasts and the molecular events of dentin formation. In this study, immortalized dental papilla mesenchymal cell lines were generated from the first mouse mandibular molars at postnatal day 3 using pSV40. These transformed cells were characterized by RT-PCR, immunohistochemistry, Western blot, and analyzed for alkaline phosphatase activity and mineralization nodule formation. One of these immortalized cell lines, iMDP-3, displayed a high proliferation rate, but retained the genotypic and phenotypic characteristics similar to primary cells as determined by expression of tooth-specific markers and demonstrated the ability to differentiate and form mineralized nodules. Furthermore, iMDP-3 cells had high transfection efficiency as well as were inducible and responded to BMP2 stimulation. We conclude that the establishment of the stable murine dental papilla mesenchymal cell line might be used for studying the mechanisms of dental cell differentiation and dentin formation. |
| Databáze: | OpenAIRE |
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