Soft texture of Atlantic salmon fillets is associated with glycogen accumulation

Autor: Mona Elisabeth Pedersen, Turid Mørkøre, Erling Olaf Koppang, Lars Helge Stien, Jacob Seilø Torgersen, Achim Kohler
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Anatomy and Physiology
Fluorescent Antibody Technique
lcsh:Medicine
Veterinary Anatomy and Physiology
Aquaculture
Extracellular matrix
chemistry.chemical_compound
Animal Musculoskeletal Anatomy
Spectroscopy
Fourier Transform Infrared
Molecular Cell Biology
Myocyte
Aggrecans
Salmo
lcsh:Science
Musculoskeletal System
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922
Principal Component Analysis
Multidisciplinary
Glycogen
Agriculture
Muscle Biochemistry
Cell biology
medicine.anatomical_structure
Biochemistry
Veterinary Diseases
Connective Tissue
Regression Analysis
Muscle
Fish Farming
Cellular Types
Research Article
Meat
Histology
Animal Types
Salmo salar
Connective tissue
Periodic acid–Schiff stain
Biology
Muscle Fibers
Collagen Type I
medicine
Animals
Muscle
Skeletal
Aggrecan
Muscle Cells
lcsh:R
biology.organism_classification
chemistry
Veterinary Science
lcsh:Q
Infrared microscopy
Heparan Sulfate Proteoglycans
Aquatic Animals
Zdroj: PLoS ONE, Vol 9, Iss 1, p e85551 (2014)
PLoS ONE
Popis: Atlantic salmon (Salmo salar L.) with soft fillets are not suited for manufacturing high quality products. Therefore fillets with insufficient firmness are downgraded, leading to severe economic losses to the farming and processing industries. In the current study, morphological characteristics of salmon fillets ranging from soft to hard were analysed. Different microscopic techniques were applied, including novel methods in this field of research: morphometric image analysis, periodic acid Schiff staining, immunofluorescence microscopy, transmission electron microscopy and fourier transform infrared microscopy. The results showed that the myocytes of soft muscle had detached cells with mitochondrial dysfunctions, large glycogen aggregates and enlarged inter cellular areas, void of extracellular matrix proteins, including lower amounts of sulfated glycoproteins. Myofibre-myofibre detachment and disappearance of the endomysium in soft muscles coincided with deterioration of important connective tissue constituents such as Collagen type I (Col I), Perlecan and Aggrecan. In summary our investigations show for the first time an association between soft flesh of Atlantic salmon and massive intracellular glycogen accumulation coinciding with degenerated mitochondria, myocyte detachment and altered extracellular matrix protein distribution. The results are important for further understanding the etiology of soft salmon.
Databáze: OpenAIRE
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