Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide
| Autor: | Chris Zueger, Byung Yang Lee, Woo-Jae Chung, Winnie S. Wong, Seung-Wuk Lee, Hyo-Eon Jin |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Phage display Receptors Peptide Chemistry Nanotubes Carbon Mechanical Engineering Cell Surface Display Techniques Diphenyl ether Bioengineering Peptide General Chemistry Plasma protein binding Biosensing Techniques Condensed Matter Physics Combinatorial chemistry chemistry.chemical_compound Click chemistry Halogenated Diphenyl Ethers General Materials Science Click Chemistry Peptides Biosensor Protein Binding |
| Zdroj: | Nano letters. 15(11) |
| ISSN: | 1530-6992 |
| Popis: | We report a highly selective and sensitive biosensor for the detection of an environmentally toxic molecule, decabrominated diphenyl ether (DBDE), one of the most common congeners of the polybrominated frame retardants (polybrominated diphenyl ether (PBDE)), using newly discovered DBDE peptide receptors integrated with carbon nanotube field-effect transistors (CNT-FET). The specific DBDE peptide receptor was identified using a high-throughput screening process of phage library display. The resulting binding peptide carries an interesting consensus binding pocket with two Trp-His/Asn-Trp repeats, which binds to the DBDE in a multivalent manner. We integrated the novel DBDE binding peptide onto the CNT-FET using polydiacetylene coating materials linked through cysteine-maleimide click chemistry. The resulting biosensor could detect the desired DBDE selectively with a 1 fM detection limit. Our combined approaches of selective receptor discovery, material nanocoating through click chemistry, and integration onto a sensitive CNT-FET electronic sensor for desired target chemicals will pave the way toward the rapid development of portable and easy-to-use biosensors for desired chemicals to protect our health and environment. |
| Databáze: | OpenAIRE |
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