Curcumin protects against stroke and increases levels of Notch intracellular domain

Autor: Sheng T. Hou, Meijuan Xiao, Zusen Ye, Man Qu, Liang Feng, Zhao Han, Rong-Yuan Zheng, Yungang Cao, Zheng Zhang, Shuang Liu
Rok vydání: 2016
Předmět:
0301 basic medicine
Doublecortin Domain Proteins
Male
Time Factors
medicine.medical_treatment
Cell Count
Pharmacology
Cerebral Ventricles
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Neurologic Examination
biology
Receptors
Notch
Neurogenesis
Brain
General Medicine
Stroke
Neuroprotective Agents
Neurology
Anesthesia
Middle cerebral artery
Microtubule-Associated Proteins
Signal Transduction
Curcumin
Doublecortin Protein
Intraperitoneal injection
Notch signaling pathway
Neuroprotection
03 medical and health sciences
medicine.artery
medicine
Animals
Analysis of Variance
business.industry
Neuropeptides
medicine.disease
Doublecortin
Rats
Disease Models
Animal
030104 developmental biology
chemistry
Bromodeoxyuridine
biology.protein
Neurology (clinical)
Nervous System Diseases
business
Reperfusion injury
030217 neurology & neurosurgery
Zdroj: Neurological research. 38(6)
ISSN: 1743-1328
Popis: To investigate whether curcumin regulates Notch signaling to cause neuroprotection and neurogenesis after focal ischemia reperfusion injury.Focal ischemia reperfusion injury was modeled in rats by occluding the middle cerebral artery. These animals were given either curcumin (300 mg/kg) or corn oil (vehicle) by intraperitoneal injection starting 1 h after stroke and continuing for 7 d. In parallel, sham-operated control animals received vehicle. All animals were killed on day 12. The different treatment groups were compared in terms of neurobehavioral deficits, BrdU incorporation, and levels of doublecortin (DCX) and Notch intracellular domain (NICD) using immunohistochemistry, immunofluorescence and Western blotting.Animals treated with curcumin showed significantly smaller neurobehavioral deficits than vehicle-treated animals after 3, 7, and 12 d of reperfusion (all p 0.05). Tissue sections from curcumin-treated animals contained significantly greater numbers of BrdU-positive cells (p 0.05) and BrdU/DCX-positive cells (p 0.01), as well as significantly higher NICD levels (p 0.01).Curcumin may protect from focal cerebral ischemia reperfusion injury as well as stimulate neurogenesis by activating the Notch signaling pathway.
Databáze: OpenAIRE
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