Effect of discriminate and indiscriminate use of oxytetracycline on residual status in broiler soft tissues

Autor: Mufsana Akter Mukta, Md. Tarek Hossain, Sayekul Hasan Khan, Kazi Rafiq, Most. Rifat Ara Ferdous, Md. Raju Ahmed, Md. Zahorul Islam, Tasnia Tabassum Anika
Rok vydání: 2020
Předmět:
Zdroj: Veterinary World, Vol 13, Iss 1, Pp 61-67 (2020)
Veterinary World
ISSN: 2231-0916
0972-8988
DOI: 10.14202/vetworld.2020.61-67
Popis: Aim: This study aimed to evaluate the effects of discriminate and indiscriminate use of oxytetracycline on hematological parameters, residual status in soft tissue of broiler and of thermal effect on oxytetracycline residual status. Materials and Methods: Eighteen, day-old male broiler chickens were purchased and were divided into three different groups (control group, discriminate group, and indiscriminate group). The control group received no antibiotics. The discriminate group received oxytetracycline 1 g/L drinking water for 5 consecutive days, and 10 days' withdrawal period was maintained before sacrifice. The indiscriminate group received oxytetracycline 1 g/L drinking water till the sacrificed day. Blood samples were collected before sacrificing for hematological analysis. After sacrificing liver, kidney, spleen, and muscle samples were collected for analysis of oxytetracycline residues in raw soft tissues. Since meat is used to cook by traditional method in Bangladesh before consumption that is why positive meat samples were cooked by traditional cooking method to evaluate the thermal effect on oxytetracycline residual status as well. Thin-layer chromatography (TLC) was done for screening of oxytetracycline residues in soft tissues. Results: Mean differences of total erythrocyte count (million/mm3), hemoglobin estimation (gm%), and packed cell volume (%) estimation were not statistically significant among the groups. TLC analysis of raw samples showed 100% positive results of all samples collected from the indiscriminate group. In contrast, samples collected from the discriminate group were negative for oxytetracycline residues. In the control group, all samples were negative for oxytetracycline residue. There was a significant (p
Databáze: OpenAIRE