Investigation of the effect of biologically active threo-Ds-isocitric acid on oxidative stress in Paramecium caudatum
| Autor: | A. N. Inozemtsev, Svetlana V. Kamzolova, Vladimir A. Samoilenko, O.V. Karpukhina, Igor G. Morgunov |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Isocitrates Antioxidant medicine.medical_treatment Yarrowia Isocitric acid Biology medicine.disease_cause 01 natural sciences Biochemistry Antioxidants 03 medical and health sciences chemistry.chemical_compound Metals Heavy 010608 biotechnology medicine Paramecium caudatum Hydrogen peroxide Hydrogen Peroxide General Medicine biology.organism_classification Ascorbic acid Culture Media Oxidative Stress 030104 developmental biology chemistry Oxidative stress Biotechnology |
| Zdroj: | Preparative Biochemistry & Biotechnology. 48:1-5 |
| ISSN: | 1532-2297 1082-6068 |
| Popis: | The effect of biologically active form (threo-Ds-) of isocitric acid (ICA) on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). ICA at concentrations between 0.5 and 10 mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of ICA was observed at its concentration 10 mM irrespective of the toxicant used (either H2O2 or heavy metal ions). ICA was found to be a more active antioxidant than ascorbic acid. Biologically active pharmaceutically pure threo-Ds-ICA was produced through cultivation of the yeast Yarrowia lipolytica and isolated from the culture liquid in the form of crystalline monopotassium salt with a purity of 99.9%. |
| Databáze: | OpenAIRE |
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