Alterations in the Gut Microbiota of Rats Chronically Exposed to Volatilized Cocaine and Its Active Adulterants Caffeine and Phenacetin
| Autor: | Juan Andrés Abin Carriquiry, Cecilia Scorza, Pablo Zunino, Claudia Piccini, Marcela Martínez Busi |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Drug media_common.quotation_subject Context (language use) Pharmacology Gut flora Toxicology digestive system Cocaine-Related Disorders Feces Random Allocation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cocaine Caffeine RNA Ribosomal 16S medicine Animals Rats Wistar media_common biology Illicit Drugs Chemistry General Neuroscience Addiction Phenacetin Biodiversity biology.organism_classification Gastrointestinal Microbiome RNA Bacterial 030104 developmental biology Toxicity Volatilization 030217 neurology & neurosurgery Central Nervous System Agents medicine.drug |
| Zdroj: | Neurotoxicity Research. 35:111-121 |
| ISSN: | 1476-3524 1029-8428 |
| DOI: | 10.1007/s12640-018-9936-9 |
| Popis: | A role of the gut microbiota in influencing brain function and emotional disorders has been suggested. However, only a few studies have investigated the gut microbiota in the context of drug addiction. Cocaine can be smoked (i.e., crack or coca paste) and its consumption is associated with a very high abuse liability and toxicity. We have recently reported that cocaine base seized samples contained caffeine and phenacetin as main active adulterants, which may potentiate its motivational, reinforcing, and toxic effects. However, the effect of volatilized cocaine and adulterants on the gut microbiota remained unknown. In the present study, we evaluated the effect of volatilized cocaine and two adulterants on the structure, diversity, and functionality of the gut microbiota in rats. Animals were chronically exposed to the fume of cocaine, caffeine, and phenacetin during 14 days. At the end of the treatment, feces were collected and the structure, composition, and functional predictions of the gut microbiota were analyzed. Cocaine significantly decreased the community richness and diversity of the gut microbiota while both cocaine and phenacetin drastically changed its composition. Phenacetin significantly increased the Firmicutes-Bacteroidetes ratio compared to the control group. When the predicted metagenome functional content of the bacterial communities was analyzed, all the treatments induced a dramatic decrease of the aromatic amino acid decarboxylase gene. Our findings suggest that repeated exposure to volatilized cocaine, as well as to the adulterants caffeine and phenacetin, leads to changes in the gut microbiota. Future studies are needed to understand the mechanisms underlying these changes and how this information may support the development of novel treatments in drug addiction. |
| Databáze: | OpenAIRE |
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