Influence of CD133+ expression on patients' survival and resistance of CD133+ cells to anti-tumor reagents in gastric cancer
| Autor: | Xiaochun Ni, Jugang Wu, Shoulian Wang, Jiwei Yu, De-Hu Chen, Bo-jian Jiang, Rui-qi Lu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
Abcg2 lcsh:RC955-962 Cell Tumor initiating cells Immunomagnetic separation Biochemistry Genetics and Molecular Biology (miscellaneous) fluids and secretions Medicine CD133 neoplasms lcsh:QH301-705.5 Cancer biology business.industry Stomach medicine.disease Molecular biology carbohydrates (lipids) medicine.anatomical_structure lcsh:Biology (General) Apoptosis Cell culture Reagent Immunology embryonic structures biology.protein cardiovascular system business |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 996-1004 (2015) |
| ISSN: | 2221-1691 |
| Popis: | Objective To investigate the influence of CD133 + expression on patients' survival and resistance of CD133 + cells to anti-tumor agents in gastric cancer (GC). Methods Influence of CD133 expression on prognosis was analyzed employing samples from patients with GC. GC cell lines were utilized to separate CD133 + and CD133 − subpopulations by immunomagnetic separation and to analyze the biological features of two subpopulations in vitro and in vivo , especially in resistant to anti-tumor reagents and its apoptotic mechanism. Results The lower CD133 + group showed a significantly better survival compared with the higher CD133 + group. The highest content of CD133 + subpopulations for KATO-III cells had stronger proliferative ability than CD133 − subpopulations. A single CD133 + cell was capable of generating new cell colony and the tumorigenicity rate in nude mice was 100% for CD133 + clonal spheres or for CD133 + cells, but 0% for CD133 − cells. Furthermore, the higher expression levels of Oct-4, Sox-2, Musashi-1 and ABCG2 in CD133 + clonal spheres were identified compared with CD133 + cells or CD133 − cells. Under the treatment of anti-tumor reagents, CD133 + cells had lower suppression rates compared with CD133 − cells while lower level of Bcl-2 and higher level of Bax were found in CD133 + cells compared with CD133 − cells. Conclusions The patients with lower CD133 + expression had a better survival. Enriched CD133 + cells in clonal sphere shared the ability to be self-renewable, proliferative, tumorigenic and resistant to anti-tumor agents as probably regulated by Bcl-2 and Bax. |
| Databáze: | OpenAIRE |
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