The Low Copy Nuclear Gene Region, Granule Bound Starch Synthase (GBSS1), as a Novel Mini-DNA Barcode for the Identification of Different Sage (Salvia) Species
| Autor: | Iffat Parveen, Sara M. Handy, Charles Wu, Jing Li, Amar G. Chittiboyina, Ikhlas A. Khan, Natascha Techen |
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| Rok vydání: | 2021 |
| Předmět: |
Nuclear gene
DNA Plant Pharmaceutical Science DNA barcoding Analytical Chemistry food Starch Synthase Drug Discovery Salvia sclarea DNA Barcoding Taxonomic Salvia Internal transcribed spacer Phylogeny Pharmacology Genetics biology Organic Chemistry Salvia officinalis biology.organism_classification food.food genomic DNA Complementary and alternative medicine biology.protein Molecular Medicine Salvia mellifera Powders Starch synthase |
| Zdroj: | Planta medica. 88(12) |
| ISSN: | 1439-0221 |
| Popis: | Morphological similarity within species makes the identification and authentication of Salvia species challenging, especially in dietary supplements that contain processed root or leaf powder of different sage species. In the present study, the species discriminatory power of 2 potential DNA barcode regions from the nuclear genome was evaluated in 7 medicinally important Salvia species from the family Lamiaceae. The nuclear internal transcribed spacer 2 and the exon 9 – 14 region of low copy nuclear gene WAXY coding for granule-bound starch synthase 1 were tested for their species discrimination ability using distance, phylogenetic, and BLAST-based methods. A novel 2-step PCR method with 2 different annealing temperatures was developed to achieve maximum amplification from genomic DNA. The granule-bound starch synthase 1 region showed higher amplification and sequencing success rates, higher interspecific distances, and a perfect barcode gap for the tested species compared to the nuclear internal transcribed spacer 2. Hence, these novel mini-barcodes generated from low copy nuclear gene regions (granule-bound starch synthase) that were proven to be effective barcodes for identifying 7 Salvia species have potential for identification and authentication of other Salvia species. |
| Databáze: | OpenAIRE |
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