Warfarin affects acute inflammatory response induced by subcutaneous polyvinyl sponge implantation in rats
| Autor: | Marina Ninkov, Aleksandra Popov Aleksandrov, Jelena Demenesku, Dina Mileusnic, Ivana Mirkov, Milena Kataranovski, Dragan Kataranovski |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cell Survival medicine.medical_treatment T cells Inflammation Pharmacology Nitric Oxide Toxicology Proinflammatory cytokine Nitric oxide Leukocyte Count 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Interferon medicine Animals Peroxidase Skin business.industry Anticoagulants General Medicine medicine.disease Rats Subcutaneous polyvinyl sponge implants 030104 developmental biology Cytokine chemistry 030220 oncology & carcinogenesis Hemostasis Immunology Cytokines Polyvinyls Tumor necrosis factor alpha Warfarin medicine.symptom business Infiltration (medical) medicine.drug |
| Zdroj: | Cutaneous and Ocular Toxicology |
| ISSN: | 1556-9535 1556-9527 |
| DOI: | 10.1080/15569527.2016.1275664 |
| Popis: | Purpose: Warfarin (WF) is an anticoagulant which also affects physiological processes other than hemostasis. Our previous investigations showed the effect of WF which gained access to the organism via skin on resting peripheral blood granulocytes. Based on these data, the aim of the present study was to examine whether WF could modulate the inflammatory processes as well. To this aim the effect of WF on the inflammatory response induced by subcutaneous sponge implantation in rats was examined. Materials and methods: Warfarin-soaked polyvinyl sponges (WF-sponges) were implanted subcutaneously and cell infiltration into sponges, the levels of nitric oxide (NO) and inflammatory cytokines tumor necrosis factor (TNF) and interleukin-6 (IL-6) production by sponge cells were measured as parameters of inflammation. T cell infiltration and cytokine interferon-γ (IFN-γ), interleukin-17 (IL-17) and interleukin-10 (IL-10) were measured at day 7 post implantation. Results: Warfarin exerted both stimulatory and suppressive effects depending on the parameter examined. Flow cytometry of cells recovered from sponges showed higher numbers of granulocytes (HIS48+ cells) at days 1 and 3 post implantation and CD11b+ cells at day 1 compared to control sponges. Cells from WF-sponges had an increased NO production (Griess reaction) at days 1 and 7. In contrast, lower levels of TNF (measured by ELISA) production by cells recovered from WF-soaked sponges were found in the early (day one) phase of reaction with unchanged levels at other time points. While IL-6 production by cells recovered from WF-soaked sponges was decreased at day 1, it was increased at day 7. Higher T cell numbers were noted in WF sponges at day 7 post implantation, and recovered cells produced more IFN-γ and IL-17, while IL-10 production remained unchanged. Conclusions: Warfarin affects some of the parameters of inflammatory reaction induced by subcutaneous polyvinyl sponge implantation. Differential (both stimulatory as well as inhibitory) effects of WF on inflammatory response to sponge implants might affect the course and/or duration of this reaction. Cutaneous and Ocular Toxicology (2017) |
| Databáze: | OpenAIRE |
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