Effects of laser polar-body biopsy on embryo quality
| Autor: | Dalit Ben-Yosef, Veronica Gold, Tamar Shwartz, Ishai Levin, Michal Shaubi, Benny Almog, Mira Malcov, Ami Amit |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty animal structures Biopsy Cleavage Stage Ovum Oocyte Retrieval DNA Fragmentation Fertilization in Vitro Polar Bodies Biology Preimplantation genetic diagnosis Andrology Human fertilization Predictive Value of Tests medicine Humans Polar body biopsy Israel Preimplantation Diagnosis Retrospective Studies Gynecology Chi-Square Distribution medicine.diagnostic_test Lasers Genetic Diseases Inborn Obstetrics and Gynecology Embryo Blastomere Oocyte Blastocyst Logistic Models medicine.anatomical_structure Reproductive Medicine Multivariate Analysis embryonic structures Female Embryo quality |
| Zdroj: | Fertility and Sterility. 97:1085-1088 |
| ISSN: | 0015-0282 |
| Popis: | Objective To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality. Study design Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy. Setting University-based tertiary-care medical center. Patient(s) Women with inherited genetics disease. Intervention(s) Laser PBB of IVF embryos for genetic diagnosis. Main Outcome Measure(s) The study and control embryos were compared for fertilization rate, pronuclear grading, and cleavage-stage parameters on days 1, 2, and 3 after oocyte retrieval. Result(s) The study embryos demonstrated higher rates of cleavage arrest (3.6% vs. 0.7%), higher rate of significant fragmentation on day 2 (9.5% vs. 3.0%), and lower rate of good cleavage embryos on day 2 (69.1% vs. 78.4%) compared with control embryos. On day 3, the study embryos had lower cleavage rates (six or more blastomeres; 56.5% vs. 74.5%), higher fragmentation (11.7% vs. 3.9%), higher rate of embryos presenting inferior cleavage pattern (57.2% vs. 38.5%), and lower mean blastomere number (5.8 ± 2.1 vs. 6.6 ± 1.9) compared with control embryos. Conclusion(s) Polar-body biopsy may have a negative effect on embryo quality. |
| Databáze: | OpenAIRE |
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