Untargeted Ultrahigh-Performance Liquid Chromatography-Hybrid Quadrupole-Orbitrap Mass Spectrometry (UHPLC-HRMS) Metabolomics Reveals Propolis Markers of Greek and Chinese Origin
Autor: | Antigoni Cheilari, Aikaterini Termentzi, Kyriaki Machera, K Stathopoulou, Konstantinos M. Kasiotis, Nektarios Aligiannis, Maria-Ioanna Stavropoulou |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
China
Uhplc hrms Pharmaceutical Science Mass spectrometry Orbitrap 01 natural sciences Article Mass Spectrometry Propolis Analytical Chemistry law.invention Chemometrics lcsh:QD241-441 Mediterranean propolis Metabolomics lcsh:Organic chemistry law Drug Discovery Chinese origin Partial least squares regression UHPLC-HRMS Physical and Theoretical Chemistry Chromatography High Pressure Liquid Chromatography Greek propolis Greece 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry chemometrics 0104 chemical sciences poplar propolis Chinese propolis Chemistry (miscellaneous) Molecular Medicine |
Zdroj: | Molecules Molecules, Vol 26, Iss 456, p 456 (2021) Volume 26 Issue 2 |
ISSN: | 1420-3049 |
Popis: | Chemical composition of propolis depends on the plant source and thus on the geographic and climatic characteristics of the site of collection. The aim of this study was to investigate the chemical profile of Greek and Chinese propolis extracts from different regions and suggest similarities and differences between them. Untargeted ultrahigh-performance liquid chromatography coupled to hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-HRMS) method was developed and 22 and 23 propolis samples from Greece and China, respectively, were analyzed. The experimental data led to the observation that there is considerable variability in terms of quality of the distinctive propolis samples. Partial least squares - discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) models were constructed and allowed the identification of significant features for sample discrimination, adding relevant information for the identification of class-determining metabolites. Chinese samples overexpressed compounds that are characteristic of the poplar type propolis, whereas Greek samples overexpress the latter and the diterpenes characteristic of the Mediterranean propolis type. |
Databáze: | OpenAIRE |
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