VAP-1/SSAO Plasma Activity and Brain Expression in Human Hemorrhagic Stroke
| Autor: | Sandra Garcia, Alan Flores, Montse Solé, Dolors Giralt, Anna Penalba, Joan Montaner, Pilar Delgado, Mercedes Unzeta, Arantxa Ortega-Aznar, Marc Ribó, Mar Hernández-Guillamon, José Alvarez-Sabín, Cristina Boada, Lidia Garcia-Bonilla |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Amine oxidase Time Factors Blotting Western Cell Inflammation Pharmacology Downregulation and upregulation Odds Ratio medicine Humans Stroke Aged Aged 80 and over chemistry.chemical_classification Intracerebral hemorrhage Chi-Square Distribution business.industry Brain Middle Aged Prognosis medicine.disease Immunohistochemistry Up-Regulation respiratory tract diseases Blot Logistic Models medicine.anatomical_structure Enzyme Neurology chemistry Spain Case-Control Studies Multivariate Analysis Female Amine Oxidase (Copper-Containing) Autopsy Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine business Cell Adhesion Molecules Intracranial Hemorrhages Biomarkers |
| Zdroj: | Cerebrovascular Diseases. 33:55-63 |
| ISSN: | 1421-9786 1015-9770 |
| DOI: | 10.1159/000333370 |
| Popis: | Background: Vascular adhesion protein-1 (VAP-1) is a cell surface and circulating enzyme that belongs to the semicarbazide-sensitive amine oxidase (SSAO) family, which oxidatively deaminates primary amines and is implicated in leukocyte extravasation. Our aim was to investigate the alteration of soluble VAP-1/SSAO activity in plasma samples after acute intracerebral hemorrhage (ICH) and its presence in human ICH brain tissue. Methods: VAP-1/SSAO activity was determined in plasma of 66 ICH patients and 58 healthy controls. In addition, we assessed the expression of VAP-1/SSAO in postmortem brain tissue from hemorrhagic stroke patients by Western blot and immunohistochemistry. Results: We observed significantly higher levels of plasma VAP-1/SSAO activity in patients with ICH compared to matched elderly controls (p = 0.001). Plasma VAP-1/SSAO activity Conclusions: Our data demonstrate that plasma VAP-1/SSAO activity is increased in ICH and predicts neurological outcome, suggesting a possible contribution of the soluble protein in secondary brain damage. Furthermore, anti-VAP-1/SSAO strategies might be a promising approach to prevent neurological worsening following ICH. |
| Databáze: | OpenAIRE |
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