The cytotoxic effects of propolis on breast cancer cells involve PI3K/Akt and ERK1/2 pathways, mitochondrial membrane potential, and reactive oxygen species generation
| Autor: | José Maurício Sforcin, Alexis Díaz-García, Hermis Rodríguez-Sánchez, Yahima Frión-Herrera, Jenny Laura Ruiz-Fuentes |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM), Tropical Medicine Institute Pedro Kouri |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Necrosis Cell Survival MAP Kinase Signaling System Cytotoxicity Immunology Apoptosis Breast Neoplasms Propolis 03 medical and health sciences chemistry.chemical_compound Phosphatidylinositol 3-Kinases 0302 clinical medicine Breast cancer Lactate dehydrogenase Cell Line Tumor medicine Cytotoxic T cell Humans Pharmacology (medical) Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Pharmacology chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species 030104 developmental biology chemistry Cancer research Female medicine.symptom Apoptosis Regulatory Proteins Reactive Oxygen Species Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T16:53:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-05-10 Propolis has been extensively used to improve health and prevent inflammatory diseases. Different types of Cuban propolis (red, brown and yellow) have been documented. The purpose of this research was to investigate the cytotoxic effects of Cuban red propolis (CP) on MDA MB-231 cell line, since breast cancer is considered one of the most common causes of mortality among women. Antiproliferative and cytotoxic activity of CP against MDA MB-231 cells were determined by the 3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyl tetrazoliumbromide (MTT) and lactate dehydrogenase (LDH) assays. Apoptosis/necrosis, involvement of PI3K/Akt and ERK1/2 pathways, mitochondrial membrane potential and expression of genes were investigated. CP extract exhibited antiproliferative and cytotoxic effects on MDA MB-231 cells, what may be probably related to PI3K/Akt and ERK1/2 pathways. A decreased expression of apoptosis-related genes (TP53, CASP3, BAX and P21) was seen, whereas the expressions of BCL-2, BCL-XL, NOXA and PUMA were unaffected. CP extract induced mitochondrial dysfunction and LDH release, what indicated cell necrosis associated with reactive oxygen species production and decreased cell migration. Our findings provide a basis for future investigation of chemopreventive and/or therapeutic studies against apoptosis-resistant breast cancer, in animals and humans. São Paulo State University (UNESP) Institute of Biosciences, Campus Botucatu Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM) Tropical Medicine Institute Pedro Kouri São Paulo State University (UNESP) Institute of Biosciences, Campus Botucatu |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|