Physiological difference between dietary obesity-susceptible and obesity-resistant Sprague Dawley rats in response to moderate high fat diet
Autor: | Dong-Hwan Shin, TaeSeok Kang, Yong Kyu Kim, JungSik Cho, Youngbuhm Huh, Kabryong Chae, Jin-Hee Hwang, JuEun Lee, DaeYeon Hwang, In-Surk Jang, ChulKyu Kim |
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Rok vydání: | 2003 |
Předmět: |
Leptin
Male medicine.medical_specialty Anabolism medicine.medical_treatment Population Receptors Cell Surface Biology Acetyl-CoA Hydrolase General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Insulin Genetic Predisposition to Disease Obesity RNA Messenger education Triglycerides DNA Primers education.field_of_study General Veterinary Triglyceride Base Sequence Cholesterol Acetyl-CoA carboxylase General Medicine Immunohistochemistry Diet Rats Endocrinology chemistry Hypothalamus Receptors Leptin Animal Science and Zoology |
Zdroj: | Experimental animals. 52(2) |
ISSN: | 1341-1357 |
Popis: | The primary aim of the present study was to define central and peripheral physiological differences between dietary obesity-susceptible (DOS) and obesity-resistant (DOR) outbred Sprague Dawley (SD) rats when given a moderate high fat diet containing 32.34% of energy as a fat. After a 9-week feeding period, the DOS-SD rats consumed significantly more feed (11.1%) and had higher abdominal (39.9%) and epididymal (27.5%) fat pads than the DOR-SD rats. In addition, serum leptin and insulin levels were significantly increased in the DOS-SD rats compared with those in the DOR-SD rats. However, we did not observe significant differences in serum triglyceride, cholesterol and glucose. No differences in hypothalamic OB-Ra and Rb mRNA expressions were found between the two groups. In contrast, arcuate NPY immunohistochemical expression was much higher in the DOS-SD rats than in the DOR-SD rats, though NPY expression in the supraoptic and paraventricular nuclei was not different between the two phenotypes. In peripheral tissues, the DOS-SD rats showed noticeably increased acetyl CoA carboxylase (ACC) mRNA expression in the liver, not epididymal fat. However, Western blot of peroxisomal proliferator activated factor gamma (PPAR gamma) in the liver and epididymal fat was not different between the two phenotypes of SD rats. It was concluded that different body weight phenotypes within outbred SD population responded differently to the development of dietary induced obesity via altered anabolic features in the hypothalamus and liver. |
Databáze: | OpenAIRE |
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