TGF‐β‐regulated tyrosine phosphatases induce lymphocyte apoptosis in Leishmania donovani‐ infected hamsters
| Autor: | Rajdeep, Banerjee, Sudeep, Kumar, Abhik, Sen, Ananda, Mookerjee, Piyali, Mukherjee, Syamal, Roy, Subrata, Pal, Pradeep, Das |
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| Rok vydání: | 2010 |
| Předmět: |
Programmed cell death
Lymphocyte Immunology Apoptosis Protein tyrosine phosphatase Lymphocyte proliferation Biology Lymphocyte Activation Gene Expression Regulation Enzymologic Transforming Growth Factor beta Cricetinae medicine Animals Immunology and Allergy Lymphocytes Enzyme Inhibitors Tyrosine Intrinsic apoptosis Cell Biology Antibodies Neutralizing Molecular biology Mitochondria Up-Regulation Cell biology medicine.anatomical_structure Leishmaniasis Visceral DNA fragmentation Female Lymph Nodes Protein Tyrosine Phosphatases Vanadates Leishmania donovani Signal Transduction |
| Zdroj: | Immunology & Cell Biology. 89:466-474 |
| ISSN: | 1440-1711 0818-9641 |
| DOI: | 10.1038/icb.2010.108 |
| Popis: | Visceral leishmaniasis, which is caused by Leishmania donovani, is one of the major health problems of the Indian subcontinent. Infected hosts have been reported to have impaired lymphoproliferation. However, the fate of anergic cells is still elusive. In the present investigation, L. donovani-infected hamsters were used to study the mechanism of lymphocyte cell death. Lymph node-derived lymphocytes were analysed for apoptotic death through mitochondrial abnormality, caspase activity and DNA degradation. The data demonstrate that the disease progression leads to a gradual impairment of lymphocyte proliferation in the presence of Concanavalin A. The fate of the anergic lymphocytes is intrinsic apoptosis, which is evident by the depolarization of the mitochondrial membrane potential, cytosolic release of cytochrome c, caspase activation and DNA fragmentation. Tumour growth factor (TGF)-β, which is secreted by macrophages, was significantly upregulated in the lymph node compartment of infected hamsters. Adding a neutralizing TGF-β antibody and a recombinant TGF-β resulted in the downregulation and induction of lymphocyte apoptosis, respectively. Furthermore, it has been observed that TGF-β triggers the apoptotic death of lymphocytes through the upregulation of tyrosine phosphatase activity and that the use of sodium orthovanadate (Na(3)VO(4), a tyrosine phosphatase inhibitor) reduces the apoptotic frequency. Thus, this study clearly reports the novel involvement of tyrosine phosphatases in TGF-β-induced lymphocyte apoptosis in Leishmania-infected hamsters. |
| Databáze: | OpenAIRE |
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