The Effect and Mechanism of Negative Pressure Wound Therapy on Lymphatic Leakage in Rabbits
| Autor: | Ying Yuan, Weidong Xiao, Xiang Hu, Yahui Niu, Aixi Yu, Baiwen Qi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment 03 medical and health sciences Lymphocele 0302 clinical medicine Dermis Negative-pressure wound therapy Edema medicine Lymphatic vessel Animals Lymphedema Lymphatic Vessels business.industry medicine.disease medicine.anatomical_structure Lymphatic system 030220 oncology & carcinogenesis Lymph Node Excision 030211 gastroenterology & hepatology Surgery Lymph Rabbits medicine.symptom Wound healing business Negative-Pressure Wound Therapy |
| Zdroj: | The Journal of surgical research. 235 |
| ISSN: | 1095-8673 |
| Popis: | Background Lymphatic leakage is one of the severe complications after lymphadenectomy. However, efficient treatment it still unclear. Materials and methods We employed inguinal lymphadenectomy and saphenous lymphatic vessel excision to establish a inguinal lymphatic leakage rabbit model. Rabbits with bilateral lymphatic leakage were divided in two groups, which were subject to negative pressure wound therapy (NPWT) on right sides and dressing change on left sides, respectively. Following 7-11 d of treatment, skin thickness and drainage volume were measured. Western blot and RT-PCR were used for analyzing the VEGF-C level. Tissues of wound were dissected and subject to anti-LYVE-1 immunohistochemical for lymphatic average positive staining area percentage and the ratio of lymphatic lumen area evaluation. Results Our lymphatic leakage model showed significant lymph stasis, delayed wound healing, and skin swelling and was confirmed by methylene blue instillation. Using this rabbit model, we found that NPWT could largely promote wound healing and resolution of skin edema. Compared with the dressing change group, the thickness of the dermis layer in the NPWT group was significantly reduced. Western blot and RT-PCR analysis showed a decrease of VEGF-C in the NPWT group. The immunohistochemical result of the NPWT group did not show a significant change in lymphatic average positive staining area percentage, whereas the ratio of lymphatic lumen area was significantly decreased, suggesting that NPWT treatment can significantly compress the dilated lymphatic vessels. Conclusions We successfully established the first clinically relevant lymphatic leakage model in rabbits. NPWT can be an effective treatment for lymphatic leakage via reducing edema and lymphatic stasis by compressing dilated lymph vessels and promoting lymphatic drainage. |
| Databáze: | OpenAIRE |
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