Dynamic colonisation by different Pneumocystis jirovecii genotypes in cystic fibrosis patients
| Autor: | F.J. Dapena, Nieves Respaldiza, Francisco J. Medrano, Jose M. Varela, F. Muñoz-Lobato, Enrique J. Calderón, C. de la Horra, M.A. Montes-Cano, Vicente Friaza, I. Mateos |
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| Jazyk: | angličtina |
| Předmět: |
Microbiology (medical)
Adult Male Adolescent Genotype Biology Pneumocystis carinii Cystic fibrosis Polymerase Chain Reaction Microbiology cystic fibrosis genotypes parasitic diseases medicine Pneumocystis jirovecii Humans molecular analysis Child Molecular Epidemiology Carriage Molecular epidemiology Pneumonia Pneumocystis Infant General Medicine Sequence Analysis DNA Ribosomal RNA medicine.disease biology.organism_classification Mitochondria Infectious Diseases RNA Ribosomal Spain Child Preschool Immunology Carrier State Female epidemiology Nested polymerase chain reaction Asymptomatic carrier |
| Zdroj: | Clinical Microbiology and Infection. (10):1008-1011 |
| ISSN: | 1198-743X |
| DOI: | 10.1111/j.1469-0691.2007.01789.x |
| Popis: | Although asymptomatic carriers of Pneumocystis jirovecii with cystic fibrosis (CF) have been described previously, the molecular epidemiology of P. jirovecii in CF patients has not yet been clarified. This study identified the distribution and dynamic evolution of P. jirovecii genotypes based on the mitochondrial large-subunit (mt LSU) rRNA gene. The mt LSU rRNA genotypes of P. jirovecii isolates in 33 respiratory samples from CF patients were investigated using nested PCR and direct sequencing. Three different genotypes were detected: 36.3% genotype 1 (85C/248C); 15.1% genotype 2 (85A/248C); 42.4% genotype 3 (85T/248C); and 6% mixed genotypes. Patients studied during a 1-year follow-up period showed a continuous colonisation/clearance cycle involving P. jirovecii and an accumulative tendency to be colonised with genotype 3. |
| Databáze: | OpenAIRE |
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