Human effector B lymphocytes express ARID3a and secrete interferon alpha
| Autor: | Judith A. James, Michelle L. Ratliff, Mikhail G. Dozmorov, Joel M. Guthridge, Graham B. Wiley, Patrick M. Gaffney, Carol F. Webb, Julie M. Ward |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunology B-Lymphocyte Subsets Alpha interferon Gene Expression Inflammation Biology Article Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Gene expression medicine Immunology and Allergy Humans Secretion Transcription factor Cells Cultured Systemic lupus erythematosus medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Interferon-alpha Dendritic Cells medicine.disease Flow Cytometry DNA-Binding Proteins 030104 developmental biology CpG site Oligodeoxyribonucleotides Toll-Like Receptor 9 Cancer research medicine.symptom 030215 immunology Transcription Factors |
| Popis: | Previously, we determined that enhanced disease activity in patients with systemic lupus erythematosus (SLE) was associated with dramatic increases in numbers of B lymphocytes expressing the transcription factor ARID3a. Our data now indicate ARID3a is important for interferon alpha (IFNa) expression and show a strong association between ARID3a expression and transcription of genes associated with lupus IFN signatures. Furthermore, both ARID3a and IFNa production were elicited in healthy control B cells upon stimulation with the TLR 9 agonist, CpG. Importantly, secretion of IFNa from ARID3a+ healthy B lymphocytes stimulated increased IFNa production in plasmacytoid dendritic cells. These data identify ARID3a+ B cells as a novel type of effector B cell, and link ARID3a expression in B lymphocytes to IFN-associated inflammatory responses in SLE. |
| Databáze: | OpenAIRE |
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