A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt

Autor: Georg Matziolis, Matthias Bungartz, Martin Schmidt, Juliane Reiche, Olaf Brinkmann, René Schiffner, Steffen Brodt
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Pathology
Tetrazolium Salts
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
Thiazolyl blue
TTC
TTC staining
osteonecrosis
vital staining
bone vitality
lcsh:QH301-705.5
Spectroscopy
General Medicine
Middle Aged
Magnetic Resonance Imaging
Computer Science Applications
medicine.anatomical_structure
Vital stain
Sodium azide
Female
Formazan
Cancellous bone
medicine.medical_specialty
Direct assessment
Bone healing
Catalysis
Article
Bone and Bones
Inorganic Chemistry
03 medical and health sciences
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
Aged
Tissue Survival
Staining and Labeling
business.industry
Organic Chemistry
Staining
030104 developmental biology
chemistry
ROC Curve
lcsh:Biology (General)
lcsh:QD1-999
business
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences; Volume 18; Issue 8; Pages: 1646
International Journal of Molecular Sciences, Vol 18, Iss 8, p 1646 (2017)
International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: 10.3390/ijms18081646
Popis: Presently, no intra-operative method for a direct assessment of bone vitality exists. Therefore, we set out to test the applicability of tetrazolium-based staining on bone samples. The explanted femoral heads of 37 patients were used to obtain either cancellous bone fragments or bone slices. Samples were stained with 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (thiazolyl blue, MTT) at different times (one to twelve hours) after explantation. Staining was quantified either spectrophotometrically after extraction of the dyes or by densitometric image analysis. TTC-staining of cancellous bone fragments and bone slices, respectively, indicated the detectability of vital cells in both types of samples in a window of up to six hours after explantation. Staining intensity at later time-points was indistinguishable from the staining of untreated samples or sodium azide treated samples, which represent dead cells. In contrast, MTT-staining of bone slices revealed intense unspecific staining, which obscured the evaluation of the vitality of the samples. The lack of a detectable increase of colour intensity in TTC-stained bone samples, which were treated more than six hours after explantation, corresponds to reduced fracture healing. The described simple procedure could provide a basis for an intraoperative decision by the orthopaedic surgeon.
Databáze: OpenAIRE
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