Deposition of extracellular matrix on silicone intraocular lens implants in rabbits
| Autor: | Yuka Okada, Yoshitaka Ohnishi, Shunsaku Ohmi, Akio Yamanaka, Michiro Kimura, Shizuya Saika, Sai-ichi Tanaka, Akira Ooshima |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty genetic structures medicine.medical_treatment Extracellular matrix component Tissue Adhesions Intraocular lens Matrix (biology) Extracellular matrix Cellular and Molecular Neuroscience chemistry.chemical_compound Silicone medicine Animals Microscopy Phase-Contrast Ultrasonography Lenses Intraocular Phacoemulsification biology Anatomy equipment and supplies Immunohistochemistry eye diseases Sensory Systems Extracellular Matrix Fibronectins Fibronectin Ophthalmology medicine.anatomical_structure chemistry Giant cell Lens (anatomy) Microscopy Electron Scanning Silicone Elastomers biology.protein Female Collagen Rabbits sense organs |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 235:517-522 |
| ISSN: | 1435-702X 0721-832X |
| DOI: | 10.1007/bf00947010 |
| Popis: | • Purpose: To examine the deposition of extracellullar matrix on silicone intraocular lenses (IOLs) implanted experimentally into rabbit eyes by electron microscopy and to determine the immunolocalization of extracellular matrix components, including collagen types and cellular fibronectin, on these IOLs. • Methods: We performed phacoemulsification and aspiration of the crystalline lens and implanted a foldable silicone IOL in the capsular bag of one eye of each of 26 adult albino rabbits under general anesthesia. After 8 weeks the animals were killed and the eyes were enucleated. The silicone IOLs were processed for electron microscopy and for immunohistochemical detection of collagen types I, III, and IV and cellular fibronectin. • Results: Electron microscopy revealed deposition of a presumed cell matrix complex on the optic portion of all silicone IOLs, as well as the adhesion of presumed macrophages and foreign-body giant cells. Cellular deposits showed immunoreactivity for cellular fibronectin. Fibrous or membranous deposits exhibited immunoreactivity for cellular fibronectin and collagen types I and III. A few type IV collagen-immunoreactive deposits were also seen. • Conclusion: Deposits of extracellular matrix components were observed on silicone IOLs. These deposits may form the scaffolding for the adhesion and proliferation of cells. These matrix components appeared to be the products of cells adhering to the surfaces of IOLs, including lens epithelial cells, macrophages and foreign-body giant cells, indicating that the process of granulation was incomplete. |
| Databáze: | OpenAIRE |
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