SPECIFICITY OF LABELLED THYMIDINE AS A DEOXYRIBONUCLEIC ACID PRECURSOR IN RADIOAUTOGRAPHY

Autor:	C. P. Leblond, M. Amano, B. Messier
Rok vydání:	1959
Předmět:	chemistry.chemical_classification Histology Nucleotides Extraction (chemistry) Nucleosides DNA Sensitivity and Specificity chemistry.chemical_compound Enzyme chemistry Biochemistry Autoradiography Anatomy Thymidine
Zdroj:	Journal of Histochemistry & Cytochemistry. 7:153-155
ISSN:	1551-5044 0022-1554
DOI:	10.1177/7.3.153
Popis:	A simple method has been devised for the extraction of deoxyribonucleic acid from histological sections by means of the enzyme DNAse without significant loss of ribonucleic acid. Removal of deoxyribonucleic acid by this method completely eliminated the radioautographic reactions observed over nuclei after injection of thymidine-H3. It is concluded that labelled thymidine is an adequate tracer of newly formed deoxyribonucleic acid in radioautographs.
Databáze:	OpenAIRE
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