The BIOSAFEPAPER project for in vitro toxicity assessments: preparation, detailed chemical characterisation and testing of extracts from paper and board samples
| Autor: | E.L. Bradley, U. Honkalampi-Hämäläinen, A. Weber, M.A. Andersson, F. Bertaud, L. Castle, O. Dahlman, P. Hakulinen, D. Hoornstra, J.-C. Lhuguenot, J. Mäki-Paakkanen, M. Salkinoja-Salonen, D.R. Speck, I. Severin, A. Stammati, L. Turco, F. Zucco, A. von Wright |
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| Přispěvatelé: | Department of Environment Food and Rural Affairs, University of Kuopio, Institute of Applied Biotechnology, KCL Science and Consulting, Department of Applied Chemistry and Microbiology [Helsinki], University of Helsinki, Centre Technique du Papier (CTP), STFI-Packforsk AB, National Public Health Institute [Finland] (THL), Laboratoire Toxicologie Alimentaire, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Istituto Superiore di Sanità, Department of Environmental and Primary Prevention, Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche [Roma] (CNR), University of Helsinki, Department of Applied Chemistry and Microbiology, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Paper
Polymers Cytotoxicity Tenax Food Contamination [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain Toxicology medicine.disease_cause Risk Assessment 01 natural sciences Gas Chromatography-Mass Spectrometry chemistry.chemical_compound 0404 agricultural biotechnology Bacillus cereus Toxicity Tests medicine Animals Humans Bioassay media_common.cataloged_instance Chemical analysis European union media_common Chromatography Ethanol Mutagenicity Tests Fatty Acids 010401 analytical chemistry Food Packaging Water Environmental Exposure 04 agricultural and veterinary sciences General Medicine BSTFA 040401 food science 0104 chemical sciences Sterols Phthalic acid chemistry Global bioassay BIOSAFEPAPER Toxicity Biological Assay Safety Gas chromatography–mass spectrometry Genotoxicity Food Science |
| Zdroj: | Food and Chemical Toxicology Food and Chemical Toxicology, Elsevier, 2008, 46 (7), pp.2498-2509. ⟨10.1016/j.fct.2008.04.017⟩ |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2008.04.017⟩ |
| Popis: | International audience; Nineteen food contact papers and boards and one non-food contact board were extracted following test protocols developed within European Union funded project BIOSAFEPAPER. The extraction media were either hot or cold water, 95% ethanol or Tenax, according to the end use of the sample. The extractable dry matter content of the samples varied from 1200 to 11,800 mg/kg (0.8-35.5 mg/dm2). According to GC-MS the main substances extracted into water were pulp-derived natural products such as fatty acids, resin acids, natural wood sterols and alkanols. Substances extracted into ethanol particularly, were diisopropylnaphthalenes, alkanes and phthalic acid esters. The non-food contact board showed the greatest number and highest concentrations of GC-MS detectable compounds. The extracts were subjected to a battery of in vitro toxicity tests measuring both acute and sublethal cytotoxicity and genotoxic effects. None of the water or Tenax extracts was positive in cytotoxicity or genotoxicity assays. The ethanol extract of the non-food contact board gave a positive response in the genotoxicity assays, and all four ethanol extracts gave positive response(s) in the cytotoxicity assays to some extent. These responses could not be pinpointed to any specific compound, although there appeared a correlation between the total amount of extractables and toxicity. |
| Databáze: | OpenAIRE |
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