Nanostructured biosensors built by layer-by-layer electrostatic assembly of enzyme-coated single-walled carbon nanotubes and redox polymers
| Autor: | Pratixa P. Joshi, David W. Schmidtke, Youdan Wang, K. L. Hobbs, Matthew B. Johnson |
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| Rok vydání: | 2006 |
| Předmět: |
Thermogravimetric analysis
Materials science Photochemistry Polymers Static Electricity Analytical chemistry Carbon nanotube Biosensing Techniques law.invention symbols.namesake Glucose Oxidase X-ray photoelectron spectroscopy Microscopy Electron Transmission law Electrochemistry General Materials Science Glucose oxidase Spectroscopy biology Nanotubes Carbon Spectrum Analysis Layer by layer Surfaces and Interfaces Condensed Matter Physics Glucose Chemical engineering biology.protein symbols Aspergillus niger Cyclic voltammetry Raman spectroscopy Biosensor Oxidation-Reduction |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 22(23) |
| ISSN: | 0743-7463 |
| Popis: | In this study, we describe the construction of glucose biosensors based on an electrostatic layer-by-layer (LBL) technique. Gold electrodes were initially functionalized with negatively charged 11-mercaptoundecanoic acid followed by alternate immersion in solutions of a positively charged redox polymer, poly[(vinylpyridine)Os(bipyridyl)2Cl(2+/3+)], and a negatively charged enzyme, glucose oxidase (GOX), or a GOX solution containing single-walled carbon nanotubes (SWNTs). The LBL assembly of the multilayer films were characterized by UV-vis spectroscopy, ellipsometry, and cyclic voltammetry, while characterization of the single-walled nanotubes was performed with transmission electron microscopy, Raman spectroscopy, thermogravimetric analysis, and X-ray photoelectron spectroscopy. When the GOX solution contained single-walled carbon nanotubes (GOX-SWNTs), the oxidation peak currents during cyclic voltammetry increased 1.4-4.0 times, as compared to films without SWNTs. Similarly the glucose electro-oxidation current also increased (6-17 times) when SWNTs were present. By varying the number of multilayers, the sensitivity of the sensors could be controlled. |
| Databáze: | OpenAIRE |
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