Korean Red Ginseng exerts anti-inflammatory and autophagy-promoting activities in aged mice
| Autor: | Jae Youl Cho, Wooram Choi, Haeyeop Kim, Chang-Kyun Han, Kon Kuk Shin, Jin Kyeong Kim, Sun Hee Hyun, Yo Han Hong, Yi-Seong Kwak |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
autophagy Inflammation Pharmacology Biochemistry Genetics and Molecular Biology (miscellaneous) TNF-α tumor necrosis factor-α 03 medical and health sciences Ginseng 0302 clinical medicine Korean Red Ginseng (KRG) RT-PCR reverse transcription polymerase chain reaction ATG autophagy-related gene medicine NF-κB nuclear factor-kappa B Kidney business.industry Monocyte Autophagy aging Botany Interleukin MCP-1 monocyte chemoattractant protein-1 IL interleukin Reverse transcription polymerase chain reaction 030104 developmental biology medicine.anatomical_structure Complementary and alternative medicine anti-inflammatory effect QK1-989 030220 oncology & carcinogenesis Tumor necrosis factor alpha medicine.symptom business AP-1 activator protein-1 Biotechnology Research Article KRG Korean Red Ginseng |
| Zdroj: | Journal of Ginseng Research Journal of Ginseng Research, Vol 45, Iss 6, Pp 717-725 (2021) |
| ISSN: | 1226-8453 |
| Popis: | Background Korean Red Ginseng (KRG) is a traditional herb that has several beneficial properties including anti-aging, anti-inflammatory, and autophagy regulatory effects. However, the mechanisms of these effects are not well understood. In this report, the underlying mechanisms of anti-inflammatory and autophagy-promoting effects were investigated in aged mice treated with KRG-water extract (WE) over a long period. Methods The mechanisms of anti-inflammatory and autophagy-promoting activities of KRG-WE were evaluated in kidney, lung, liver, stomach, and colon of aged mice using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), and western blot analysis. Results KRG-WE significantly suppressed the mRNA expression levels of inflammation-related genes such as interleukin (IL)-1β, IL-8, tumor necrosis factor (TNF)-α, monocyte chemoattractant protein-1 (MCP-1), and IL-6 in kidney, lung, liver, stomach, and colon of the aged mice. Furthermore, KRG-WE downregulated the expression of transcription factors and their protein levels associated with inflammation in lung and kidney of aged mice. KRG-WE also increased the expression of autophagy-related genes and their protein levels in colon, liver, and stomach. Conclusion The results suggest that KRG can suppress inflammatory responses and recover autophagy activity in aged mice. Graphical abstract Image 1 |
| Databáze: | OpenAIRE |
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