Applying pollen DNA metabarcoding to the study of plant-pollinator interactions
| Autor: | Julie Fowler, Berry J. Brosi, Emily K. Dobbs, Brice Lawley, Kevin S. Burgess, Connor Morozumi, Karen L. Bell, David L. Gruenewald |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Application Article Pollination rbcL Plant Science Biology medicine.disease_cause 010603 evolutionary biology 01 natural sciences pollination networks DNA metabarcoding 03 medical and health sciences chemistry.chemical_compound Data sequences Pollinator Pollen Botany medicine palynology Ecology Evolution Behavior and Systematics Palynology plant–pollinator interactions Illumina miseq 030104 developmental biology chemistry Taxonomic resolution ITS DNA |
| Zdroj: | Applications in Plant Sciences |
| ISSN: | 2168-0450 |
| Popis: | Premise of the study: To study pollination networks in a changing environment, we need accurate, high-throughput methods. Previous studies have shown that more highly resolved networks can be constructed by studying pollen loads taken from bees, relative to field observations. DNA metabarcoding potentially allows for faster and finer-scale taxonomic resolution of pollen compared to traditional approaches (e.g., light microscopy), but has not been applied to pollination networks. Methods: We sampled pollen from 38 bee species collected in Florida from sites differing in forest management. We isolated DNA from pollen mixtures and sequenced rbcL and ITS2 gene regions from all mixtures in a single run on the Illumina MiSeq platform. We identified species from sequence data using comprehensive rbcL and ITS2 databases. Results: We successfully built a proof-of-concept quantitative pollination network using pollen metabarcoding. Discussion: Our work underscores that pollen metabarcoding is not quantitative but that quantitative networks can be constructed based on the number of interacting individuals. Due to the frequency of contamination and false positive reads, isolation and PCR negative controls should be used in every reaction. DNA metabarcoding has advantages in efficiency and resolution over microscopic identification of pollen, and we expect that it will have broad utility for future studies of plant–pollinator interactions. |
| Databáze: | OpenAIRE |
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