Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere

Autor: Anam Nasir, Waqar Ahmad, Fazal Sattar, Iram Ashfaq, Stephen R. Lindemann, Ming-Hsu Chen, Wim Van den Ende, Ebru Toksoy Ӧner, Onur Kirtel, Shazia Khaliq, Muhammad A. Ghauri, Munir A. Anwar
Rok vydání: 2022
Předmět:
Zdroj: Antonie van Leeuwenhoek. 115:1101-1112
ISSN: 1572-9699
0003-6072
DOI: 10.1007/s10482-022-01760-6
Popis: A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2 → 6) linked backbone with β(2 → 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × 10
Databáze: OpenAIRE