The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile Flow and Cyclic Strain, In Vitro
| Autor: | Jonathan P. Gertler, Gilbert L'Italien, Christopher J. Kwolek, Aziz Benbrahim, Roslyn W. Orkin, Nancy Conroy, Olga Tsukurov, William M. Abbott, Barbara B. Milinazzo |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
medicine.medical_specialty Intimal hyperplasia Nitric Oxide Synthase Type III Pulsatile flow Gene Expression Hemodynamics Tissue plasminogen activator Internal medicine Plasminogen Activator Inhibitor 1 medicine Humans Saphenous Vein RNA Messenger Messenger RNA Endothelin-1 biology business.industry General Medicine Anatomy medicine.disease In vitro Endothelial stem cell Nitric oxide synthase Endocrinology Pulsatile Flow Tissue Plasminogen Activator biology.protein Surgery Endothelium Vascular Stress Mechanical Nitric Oxide Synthase Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Annals of Vascular Surgery. 14:260-267 |
| ISSN: | 0890-5096 |
| DOI: | 10.1007/s100169910044 |
| Popis: | Adult human saphenous vein endothelial cells (HVEC) were cultured in a compliant tubular device and evaluated by Northern hybridization for the effects of combined pressurized pulsatile flow and cyclic strain on the expression of mRNAs for endothelin-1 (ET-1), endothelial cell nitric oxide synthase (ecNOS), tissue plasminogen activator (tPA), and plasminogen activator inhibitor type 1 (PAI-1). The hemodynamic environment was designed to mimic shear stress conditions at the distal anastomosis of a saphenous vein graft, a common site of intimal proliferation. Steady-state mRNA levels in experimental tubes were expressed relative to that in controls. No changes were observed in ET-1 mRNA after 1 and 24 hr, but a 50% decrease in experimental cultures was observed after 48 hr in the vascular simulating device. Similar results were obtained for ecNOS mRNA, although a subgroup (4 of 11) showed a significant decrease (>50%) by 24 hr. For tPA mRNA, no change was observed after 1 hr, but a significant decrease (>60%) was measured after 24 hr and no message was detectable after 48 hr. Steady-state levels for PAI-1 mRNA remained unchanged through 48 hr of treatment. These results show that pressure, pulsatile flow, and cyclic strain, when applied in concert, differentially alter vasoactive and fibrinolytic functions in HVEC. Moreover, the dramatic decrease in steady-state levels of tPA mRNA is consistent with a shift toward an increased thrombotic state. |
| Databáze: | OpenAIRE |
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