Planar cell polarity induces local microtubule bundling for coordinated ciliary beating
| Autor: | Kazuhiro Oiwa, Satoshi Konishi, Yasuo Imoto, Shogo Nakayama, Toshinori Namba, Masahide Takahashi, Atsushi Tamura, Tomoki Nishida, Ken'ya Furuta, Maki Takagishi, Tomoki Yano, Sachiko Tsukita, Elisa Herawati, Shuji Ishihara |
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| Rok vydání: | 2020 |
| Předmět: |
Mucociliary clearance
macromolecular substances Biology Development Cell junction Microtubules Article 03 medical and health sciences Mice 0302 clinical medicine Microtubule Cell polarity Planar cell polarity Basal body Animals Cilia Process (anatomy) Cytoskeleton 030304 developmental biology Mice Knockout 0303 health sciences urogenital system Cell Polarity Epithelial Cells Cell Biology Basal Bodies Trachea Tubulin Mucociliary Clearance Biophysics biology.protein Carrier Proteins 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 |
| Popis: | Nakayama et al. show that Daple is responsible for the increased microtubule (MT) density on the Frizzled side of the apical junctional complex (AJC) in multiciliated cells via bundling and stabilizing MTs and mediating interactions between MTs and the AJC. Multiciliated cells (MCCs) in tracheas generate mucociliary clearance through coordinated ciliary beating. Apical microtubules (MTs) play a crucial role in this process by organizing the planar cell polarity (PCP)–dependent orientation of ciliary basal bodies (BBs), for which the underlying molecular basis remains elusive. Herein, we found that the deficiency of Daple, a dishevelled-associating protein, in tracheal MCCs impaired the planar polarized apical MTs without affecting the core PCP proteins, causing significant defects in the BB orientation at the cell level but not the tissue level. Using live-cell imaging and ultra-high voltage electron microscope tomography, we found that the apical MTs accumulated and were stabilized by side-by-side association with one side of the apical junctional complex, to which Daple was localized. In vitro binding and single-molecule imaging revealed that Daple directly bound to, bundled, and stabilized MTs through its dimerization. These features convey a PCP-related molecular basis for the polarization of apical MTs, which coordinate ciliary beating in tracheal MCCs. |
| Databáze: | OpenAIRE |
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