Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro
| Autor: | Gordon S. Mitchell, Stephen M. Johnson, Michael R. Wenninger, Julia E. R. Wilkerson |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pulmonary and Respiratory Medicine
Agonist Hypoglossal Nerve medicine.medical_specialty Time Factors Physiology medicine.drug_class Biguanides Action Potentials In Vitro Techniques Standard solution Biology Internal medicine Respiration medicine Animals Respiratory system Respiratory Burst Dose-Response Relationship Drug General Neuroscience Anatomy Respiratory activity In vitro Culture Media Serotonin Receptor Agonists Turtles Endocrinology Control of respiration sense organs Brain Stem Phenylbiguanide |
| Zdroj: | Respiratory Physiology & Neurobiology. 138:253-263 |
| ISSN: | 1569-9048 |
| DOI: | 10.1016/j.resp.2003.08.006 |
| Popis: | Our goal was to determine whether time-dependent changes in respiratory motor output in vitro could be minimized by altering bath solution composition. Adult turtle brainstems were bathed in standard solution, nutrient-rich Dulbecco's Eagle media (100 or 25% concentration), or standard solution with phenylbiguanide (PBG, 5-HT3 agonist which increases respiratory drive). Except for a 63% frequency increase in PBG solution, hypoglossal bursts were unaltered within 100 min of observation. Respiratory activity was abolished within 7 h in 100% Dulbecco's compared with a mean of 24-31 h in other test solutions. At 12 h, burst frequency decreased faster in standard solution and 25% Dulbecco's (-0.28+/-0.07 and -0.13+/-0.05 bursts/h, respectively) compared with PBG solution (-0.09+/-0.04 bursts/h); amplitude declined at approximately 2%/h in all solutions. The tendency for episodic discharge decreased gradually in standard solution, but was eliminated in 25% Dulbecco's and PBG solution. Certain bath solutions may minimize time-dependent frequency reductions but may also cause breathing pattern changes. |
| Databáze: | OpenAIRE |
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