OSTEOPONTIN ANTISENSE OLIGONUCLEOTIDE INHIBITS ADHESION OF CALCIUM OXALATE CRYSTALS IN MADIN-DARBY CANINE KIDNEY CELL
| Autor: | T. Kurita, Kenjiro Kohri, T. Yamate, T. Umekawa, M. Iguchi |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
biology medicine.diagnostic_test business.industry Oligonucleotide Urology Madin Darby canine kidney cell Adhesion Immunofluorescence Molecular biology Endocrinology stomatognathic system Calcium Oxalate Crystals Internal medicine Antisense oligonucleotides Sense (molecular biology) biology.protein Medicine Osteopontin business |
| Zdroj: | The Journal of Urology. :1506-1512 |
| ISSN: | 0022-5347 |
| DOI: | 10.1097/00005392-199810000-00106 |
| Popis: | Purpose: We previously suggested that osteopontin (OPN) plays an important role in the process of deposited calcium crystals adhesion to cells in the early stages of urolithiasis. To further confirm this theory, we tried to inhibit OPN expression at the translational level and examined its cellular biological consequence on the formation and adhesion process of crystals.Materials and Methods: We synthesized antisense and sense oligonucleotide corresponding to an appropriate part of the coding sequence for OPN in Madin Darby canine kidney (MDCK) cells. With the aid of lipofection reagent DOTAP, antisense and sense oligonucleotide introduced into MDCK cells grown in a confluent monolayer. After further incubation, inhibition of OPN expression in the cells was assessed by immunofluorescence photomicrography, and formation of calcium oxalate crystals was quantitated by incorporation of45 Ca into the stone and visualized by scanning electron microscopy (SEM).Results: Antisense oligonucleotide at concen... |
| Databáze: | OpenAIRE |
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