Characterization of rubella virus-specific antibody responses by using a new synthetic peptide-based enzyme-linked immunosorbent assay
| Autor: | Ting Zhang, Martial Lacroix, Maan Zrein, Margaret Ho, Leslie Ann Mitchell, Diane Decarie, Aubrey J. Tingle |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Adult Male Adolescent medicine.drug_class Molecular Sequence Data Rubella Syndrome Congenital Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Monoclonal antibody Antibodies Viral Rubella Immunoglobulin G Epitope Rubella vaccine Antibody Specificity Neutralization Tests medicine Humans Rubella Vaccine Amino Acid Sequence Child Hemagglutination assay Rubella virus Hemagglutination Inhibition Tests medicine.disease Virology Peptide Fragments biology.protein Female Antibody medicine.drug Research Article |
| Zdroj: | Europe PubMed Central |
| Popis: | Rubella virus (RV)-specific immunoglobulin G antibodies were studied by enzyme-linked immunosorbent assay (ELISA) techniques in sera from RV (RA 27/3)-vaccinated individuals, patients experiencing natural RV infection, congenital rubella syndrome patients, and individuals failing to respond to repeated RV immunization. Results obtained by using whole-RV ELISAs (detergent-solubilized M33 strain or intact Gilchrist strain) and hemagglutination inhibition (HAI) and neutralization (NT) assays were compared with results obtained with the same sera by using ELISAs employing a synthetic peptide, BCH-178, representing a putative neutralization domain on the RV E1 protein. Murine RV E1-specific monoclonal antibodies with HAI and NT activities exhibited strong reactivity in ELISAs with BCH-178 peptide. In sera from RA 27/3-vaccinated individuals collected at 0 (prevaccine), 1, 2, 3, 4, 5, 6, 12, and 24 to 52 weeks postvaccine, the development of E1-peptide-reactive antibodies closely paralleled increases in RV-specific antibodies measured by whole-RV ELISAs and HAI and NT assays. Similarly, sequential serum samples obtained from patients during acute and convalescent phases of natural RV infection showed a coordinate increase in RV-specific antibodies as measured by whole-RV and peptide ELISAs. Conversely, congenital rubella syndrome patient sera, although exhibiting high levels of antibody in whole-RV ELISAs, had little or no antibody directed to the neutralization domain peptide. Sera from patients failing to respond to repeated RV immunization contained very low levels of RV-specific antibody in all ELISAs. Our results that the sequence represented by BCH-178 peptide may be a previously unidentified neutralization epitope for human antibodies on the RV E1 protein and may prove useful in determining effective RV immunity. |
| Databáze: | OpenAIRE |
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