Characterization of Different Types of Excitability in Large Somatosensory Neurons and Its Plastic Changes in Pathological Pain States

Autor: Rou-Gang Xie, San-Jue Hu, Chu Wenguang, Ceng Luo
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
excitability type
Action Potentials
Sensory system
hyperpolarization-activated cation current
Biology
Somatosensory system
Catalysis
Article
lcsh:Chemistry
Inorganic Chemistry
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Dorsal root ganglion
Ganglia
Spinal
medicine
Animals
Patch clamp
Neurons
Afferent
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Pathological
Spectroscopy
Cells
Cultured
Neuronal Plasticity
large DRG neurons
Organic Chemistry
General Medicine
Sensory neuron
Computer Science Applications
Rats
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
nervous system
chronic compression of dorsal root ganglion
Peripheral nerve injury
Neuralgia
Neuroscience
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 161
International Journal of Molecular Sciences, Vol 19, Iss 1, p 161 (2018)
ISSN: 1422-0067
Popis: Sensory neuron types have been distinguished by distinct morphological and transcriptional characteristics. Excitability is the most fundamental functional feature of neurons. Mathematical models described by Hodgkin have revealed three types of neuronal excitability based on the relationship between firing frequency and applied current intensity. However, whether natural sensory neurons display different functional characteristics in terms of excitability and whether this excitability type undergoes plastic changes under pathological pain states have remained elusive. Here, by utilizing whole-cell patch clamp recordings, behavioral and pharmacological assays, we demonstrated that large dorsal root ganglion (DRG) neurons can be classified into three classes and four subclasses based on their excitability patterns, which is similar to mathematical models raised by Hodgkin. Analysis of hyperpolarization-activated cation current (Ih) revealed different magnitude of Ih in different excitability types of large DRG neurons, with higher Ih in Class 2-1 than that in Class 1, 2-2 and 3. This indicates a crucial role of Ih in the determination of excitability type of large DRG neurons. More importantly, this pattern of excitability displays plastic changes and transition under pathological pain states caused by peripheral nerve injury. This study sheds new light on the functional characteristics of large DRG neurons and extends functional classification of large DRG neurons by integration of transcriptomic and morphological characteristics.
Databáze: OpenAIRE
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