Effects of electroacupuncture with different frequencies on spinal ionotropic glutamate receptor expression in complete Freund's adjuvant-injected rat
| Autor: | Jin Kang, Byung Tae Choi, Un-Bock Jo |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Pain Threshold medicine.medical_specialty Histology Calcitonin Gene-Related Peptide Freund's Adjuvant Stimulation Cell Count Calcitonin gene-related peptide Zusanli Rats Sprague-Dawley Internal medicine medicine Animals Edema Receptor Injections Spinal Pain Measurement Inflammation Chemistry Foot Glutamate receptor Cell Biology General Medicine Anatomy Spinal cord Immunohistochemistry Rats medicine.anatomical_structure Endocrinology Electroacupuncture Receptors Glutamate Spinal Cord Ionotropic glutamate receptor Proto-Oncogene Proteins c-fos Ionotropic effect |
| Zdroj: | Acta histochemica. 107(1) |
| ISSN: | 0065-1281 |
| Popis: | We investigated expressional changes of spinal glutamate receptors by electroacupuncture (EA) in an inflammatory animal model. Inflammation was induced by an intraplantar injection of complete Freund's adjuvant (CFA) into the hindpaw of male Sprague-Dawley rats. Bilateral EA stimulation at 2, 15 and 120 Hz was applied at those acupoints corresponding to Zusanli and Sanyinjiao in man using needles with 3-day intervals for 30 days. Paw edema and mechanical thresholds were measured by a water displacement plethysmometer and Analgesy-Meter, respectively. Edema and mechanical sensitivity of the hindpaw induced by CFA-injection were strongly inhibited by EA stimulation. At 30 days after CFA-injection, effects of EA on ionotropic glutamate receptor (NR-1, NR-2A, GluR-1 and GluR-2/3) expression in association with c-fos and calcitonin gene-related peptide (CGRP) expression were observed in the dorsal horn of the spinal cord using immunohistochemistry. The number of c-fos-like immunostained cells was decreased significantly in the superficial laminae of the dorsal horn by 2Hz EA, but CGRP expression also showed a marked decrease in the same region using the other types of EA stimulation. N-methyl-D-aspartate receptor (NR-1 and NR-2A) expression was attenuated in all regions of the dorsal horn by all types of EA. Of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (GluR-1 and -2/3), only GluR-1 expression was prevented by EA treatment in the superficial laminae and the neck of the dorsal horn. It is concluded that EA treatment can attenuate inflammatory edema and mechanical thresholds in CFA-injected rats through modulating expression of ionotropic glutamate receptors, and especially N-methyl-D-aspartate receptors, in the dorsal horn of the spinal cord. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect