Laboratory Diagnosis of HEV Infection
| Autor: | Youchun Wang, Chenyan Zhao |
|---|---|
| Rok vydání: | 2023 |
| Předmět: |
0301 basic medicine
biology Igm antibody business.industry viruses virus diseases Acute infection Anti hev igm medicine.disease_cause Virology digestive system diseases Serology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Hepatitis E virus Antigen Hev antigen medicine biology.protein 030211 gastroenterology & hepatology Antibody business |
| Zdroj: | Advances in Experimental Medicine and Biology ISBN: 9789819913039 Advances in Experimental Medicine and Biology ISBN: 9789402409406 |
| DOI: | 10.1007/978-981-99-1304-6_14 |
| Popis: | Serological and nucleic acid tests for detecting hepatitis E virus (HEV) have been developed for both epidemiologic and diagnostic purposes. The laboratory diagnosis of HEV infection depends on the detection of HEV antigen, HEV RNA, and serum antibodies against HEV (immunoglobulin [Ig]A, IgM, and IgG). Anti-HEV IgM antibodies can be detected during the acute phase of the illness and can last approximately 4 or 5 months, representing recent exposure, whereas anti-HEV IgG antibodies can last more than 10 years, representing remote exposure. Thus, the diagnosis of acute infection is based on the presence of anti-HEV IgM, HEV antigen, and HEV RNA, while epidemiological investigations are mainly based on anti-HEV IgG. Although significant progress has been made in developing and optimizing different formats of HEV assays, improving their sensitivity and specificity, there are many shortcomings and challenges in inter-assay concordance, validation, and standardization. This article reviews the current knowledge on the diagnosis of HEV infection, including the most common available laboratory diagnostic techniques. |
| Databáze: | OpenAIRE |
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