Prospects of molecular markers in Fusarium species diversity
| Autor: | Nayaka S. Chandra, Harischandra Sripathy Prakash, E. G. Wulff, B. P. Nandini, C. N. Mortensen, A. C. Udayashankar, Siddapura Ramachandrappa Niranjana |
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| Rok vydání: | 2011 |
| Předmět: |
Fusarium
Molecular Sequence Data Genomics Biology Applied Microbiology and Biotechnology DNA barcoding Genome Fungal Proteins chemistry.chemical_compound Molecular marker Humans Genetic variability Mycological Typing Techniques Phylogeny Plant Diseases Genetics Genetic Variation food and beverages Species diversity General Medicine biology.organism_classification Mycoses chemistry Evolutionary biology Pyrosequencing Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 90:1625-1639 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-011-3209-3 |
| Popis: | Recent developments in genomics have opened up for newer opportunities to study the diversity and classification of fungi. The genus Fusarium contains many plant pathogens that attack diverse agricultural crops. Fusarium spp. are not only pathogenic to plants but are also known as toxin producers that negatively affect animal and human health. The identification of Fusarium species still remains one of the most critical issues in fungal taxonomy, given that the number of species recognized in the genus has been constantly changing in the last century due to the different taxonomic systems. This review focuses of various molecular-based techniques employed to study the diversity of Fusarium species causing diseases in major food crops. An introduction of fusarial diseases and their mycotoxins and molecular-marker-based methods for detection introduce the concept of marker application. Various well-known molecular techniques such as random amplified polymorphic DNA, amplification fragment length polymorphism, etc. to more modern ones such as DNA microarrays, DNA barcoding, and pyrosequencing and their application form the core of the review. Target regions in the genome which can be potential candidates for generation of probes and their use in phylogeny of Fusarium spp. are also presented. The concluding part emphasizes the value of molecular markers for assessing genetic variability and reveals that molecular tools are indispensable for providing information not only of one Fusarium species but on whole fungal community. This will be of extreme value for diagnosticians and researchers concerned with fungal biology, ecology, and genetics. |
| Databáze: | OpenAIRE |
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