Resveratrol promotes an inhibitory effect on the turbot scuticociliate parasite Philasterides dicentrarchi by mechanisms related to cellular detoxification
| Autor: | Pedro Morais, Jesús Lamas, M. L. Sanmartín, J.A. Arranz, Francisco Orallo, José Leiro |
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| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Antioxidant medicine.medical_treatment Cellular detoxification Antiprotozoal Agents Apoptosis medicine.disease_cause Antioxidants Superoxide dismutase chemistry.chemical_compound Oxygen Consumption Stilbenes medicine Animals Ciliophora chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug General Veterinary biology General Medicine Glutathione Ascorbic acid Mitochondria Biochemistry chemistry Resveratrol Catalase Flatfishes biology.protein Parasitology Reactive Oxygen Species Oxidative stress |
| Zdroj: | Veterinary Parasitology. 161:307-315 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2008.12.025 |
| Popis: | The mechanisms involved in antiparasitic activity of the natural nonflavonoid polyphenol resveratrol (RESV) on the turbot (Psetta maxima) scuticoliate parasite Philasterides dicentarchi were investigated. At concentrations higher than 50microM, RESV caused significant inhibition of the in vitro growth of the ciliates, which was apparent on the third day of culture and, at the same concentration, RESV caused significant inhibition of O(2) consumption. RESV, at a concentration of 100microM, produced a significant increase in the production of intracellular reactive oxygen species (ROS), which were inhibited by the addition of 1mM of L (+) ascorbic acid. RESV (100microM) also caused significant inhibition of peroxidase, catalase and superoxide dismutase activities, but stimulated the activity of the redox regulating enzyme glutathione S-transferase. Confocal microscopy with the mitochondria-sensitive dye MitoTracker Orange CMTMRos revealed that RESV at concentrations higher than 50microM significantly increased the levels of fluorescence inside mitochondria and, at the same concentration, also caused an increase in the vacuolization of the trophozoites. The results obtained in the present study suggest that the inhibitory activity of RESV on the ciliate P. dicentrarchi is related to the induction of oxidative stress and to the inability of the parasite to eliminate ROS as a result of modified activity of antioxidant enzymes. |
| Databáze: | OpenAIRE |
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