Development of novel fluorescent particles applicable for phagocytosis assays with human macrophages
| Autor: | Cecilia Sóñora, Paula Arbildi, Iris Miraballes-Martínez, Ana Hernández |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemistry Phagocytosis Macrophages Clinical Biochemistry Immunology 02 engineering and technology 021001 nanoscience & nanotechnology Flow Cytometry Fluorescence Molecular biology Microspheres Cell biology Cell Line 03 medical and health sciences Medical Laboratory Technology 030104 developmental biology Fluorescent microspheres Apoptosis Immunology and Allergy Humans Particle Size 0210 nano-technology Fluorescent Dyes |
| Zdroj: | Journal of immunoassayimmunochemistry. 39(1) |
| ISSN: | 1532-4230 |
| Popis: | Phagocytosis is a fundamental process for removal of pathogens and for clearance of apoptotic cells. The objective of this work was the preparation of fluorescent microspheres by a simple method and the evaluation of its applicability in phagocytosis assays by using different human derived cells, differentiated THP-1 cell line and blood monocytes, with flow cytometry measurements for functionality assays. Our results show that microparticles are efficiently internalised in a non-opsonised form and in dose-dependent manner by both cellular types. Concerning mechanism we determined that tTG-β3 integrin signaling could be involved in the uptake of these particles. |
| Databáze: | OpenAIRE |
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