Detection and cytotoxicity of cisplatin-induced superoxide anion in monolayer cultures of a human ovarian cancer cell line
| Autor: | Toshiko Tanaka, Mariko Naito, Hisamitsu Tamai, Masahiro Tateishi, Hidetaka Masuda |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
medicine.medical_specialty endocrine system diseases Cell Survival Antineoplastic Agents Toxicology medicine.disease_cause law.invention Superoxide dismutase chemistry.chemical_compound Superoxides law Internal medicine Tumor Cells Cultured Extracellular medicine Humans Pharmacology (medical) Cytotoxicity Chemiluminescence Ovarian Neoplasms Pharmacology Cisplatin biology Superoxide Dismutase Chemistry Superoxide Imidazoles Molecular biology Endocrinology Oncology Cell culture Pyrazines Luminescent Measurements biology.protein Female Oxidative stress medicine.drug |
| Zdroj: | Cancer Chemotherapy and Pharmacology. 47:155-160 |
| ISSN: | 1432-0843 0344-5704 |
| DOI: | 10.1007/s002800000198 |
| Popis: | Superoxide anions (O2-) generated by cisplatin [cis-diamminedichloroplatinum (II), DDP] were determined by measuring the chemiluminescence from the luminescence probe, 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (methyl Cypridina luciferin analog, MCLA), in monolayer cultures of a human ovarian cancer cell line (A2780) in physiological saline at pH 7.0. In a time-course study, chemiluminescence of MCLA (C-MCLA) showed a peak level at 10 min and a background level at 60 min after the addition of DDP. The intensity of C-MCLA increased with increasing concentrations of DDP or MCLA in a limited concentration range, and was significantly correlated (r = 0.960) with the number of A2780 cells. DDP-induced C-MCLA was completely inhibited by the addition of the O2- scavenger, superoxide dismutase (SOD). However, SOD did not decrease DDP cytotoxicity in terms of clonogenic cell survival. These findings suggest that DDP generates extracellular O2-, probably by interaction with the cellular membrane in A2780 cells, and O2- does not lead to cellular damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink