Automated column-switching high-performance liquid chromatography method for the determination of 1-hydroxypyrene in human urine
| Autor: | Yves Morele, P Delsaut, T Nicot, P. Simon |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Analyte Pyrenes Chromatography Chemistry Hydrolysis Metabolite General Chemistry Urine High-performance liquid chromatography Automation chemistry.chemical_compound Glucuronides Spectrometry Fluorescence Column chromatography Hydrocarbon Calibration Humans Centrifugation Quantitative analysis (chemistry) Chromatography High Pressure Liquid Mutagens |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 732:91-101 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(99)00270-4 |
| Popis: | An on-line sample treatment method to determine 1-hydroxypyrene (1-OHP), a metabolite of polycyclic aromatic hydrocarbons (PAHs), in human urine has been developed. The hydrolysed biological fluid was directly injected into the chromatographic system after only centrifugation. A miniature precolumn loop packed with a preparative phase and coupled on-line to a liquid chromatographic (LC) system was used for analyte enrichment. The analytes were non-selectively desorbed with the LC eluent and cleaned by means of a column-switching procedure comprising two purification columns and an analytical column. Pre-treatment and analysis were performed within 2 and 20 min, respectively. Average 1-OHP recovery reached 99% in the 1–25 μg/l range of urine, and the quantitation limit was 20 ng/l for 100 μl of injected sample. A comparison with a more time-consuming off-line method was performed by analysing 120 urine samples of PAH-exposed and expected unexposed workers; the statistical treatment indicated that both methods are in agreement. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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