In vitro and in silico inhibition properties of fucoidan against α-amylase and α-D-glucosidase with relevance to type 2 diabetes mellitus
| Autor: | Chandrasekaran Raghu, H A Arjun, S. Lakshmana Senthil, P Anantharaman |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Polymers and Plastics
Protein Conformation In silico 02 engineering and technology 010402 general chemistry 01 natural sciences Fucose chemistry.chemical_compound Structure-Activity Relationship Pharmacokinetics Polysaccharides Materials Chemistry Computer Simulation Glycoside Hydrolase Inhibitors Amylase IC50 biology Chemistry Fucoidan Organic Chemistry alpha-Glucosidases 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Molecular Docking Simulation Biochemistry Diabetes Mellitus Type 2 Docking (molecular) biology.protein alpha-Amylases 0210 nano-technology |
| Zdroj: | Carbohydrate polymers. 209 |
| ISSN: | 1879-1344 |
| Popis: | This study investigated the in vitro and in silico inhibitory properties of fucoidan extracted from Turbinaria conoides against α-amylase and α-D-glucosidase. Extracted fucoidan contained 59 and 35% of fucose and sulphate and was characterized using 1H NMR. Dose dependent inhibition assays showed maximum of 85 and 72% of inhibition for α-amylase and α-D-glucosidase at 2.07 and 1.03 μM concentration of fucoidan. The IC50 value of fucoidan was found to be 1.07 and 0.68 μM against α-amylase and α-D-glucosidase. In silico studies (grid based docking) by Schrodinger software revealed that fucoidan as a potent inhibitor for both α-amylase and α-D-glucosidase based on number of interactions, hydrogen bond length and binding energy. Furthermore, fucoidan fulfilled the pharmacokinetic properties thus promising to develop fucoidan as drug for type 2 DM. |
| Databáze: | OpenAIRE |
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