Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities

Autor: Alexis Bazire, Dominique Haras, Carlos Blanco, Théophile Bernard, Mohamed Jebbar, Farès Diab
Přispěvatelé: Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Microbiologie : Risques Infectieux, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CHU Pontchaillou [Rennes]-Faculté de Chirurgie Dentaire de Rennes-Faculté d'Odontologie-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-CHU Pontchaillou [Rennes]-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes - UFR d'Odontologie (UR Odontologie), Université de Rennes (UR)-Université de Rennes (UR), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
MESH: Sodium Chloride
Proteome
[SDV]Life Sciences [q-bio]
Catabolite repression
Succinic Acid
MESH: Enzymes
Ectoine
Sodium Chloride
Microbiology
Enzyme Repression
MESH: Enzyme Repression
03 medical and health sciences
chemistry.chemical_compound
Betaine
Serine dehydratase
MESH: Osmolar Concentration
Bacterial Proteins
Electrophoresis
Gel
Two-Dimensional
MESH: Bacterial Proteins
030304 developmental biology
0303 health sciences
MESH: Gene Expression Regulation
Bacterial
030306 microbiology
Catabolism
Chemistry
Osmolar Concentration
Gene Expression Regulation
Bacterial
MESH: Electrophoresis
Gel
Two-Dimensional
Adaptation
Physiological
MESH: Adaptation
Physiological
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Enzymes
MESH: Succinic Acid
MESH: Glucose
MESH: Proteome
Glucose
Biochemistry
Glycine
Pseudomonas aeruginosa
MESH: Pseudomonas aeruginosa
MESH: Betaine
Energy source
Zdroj: Microbiology
Microbiology, Microbiology Society, 2006, 152 (Pt 5), pp.1395-406. ⟨10.1099/mic.0.28652-0⟩
Microbiology (Reading, England)
Microbiology (Reading, England), 2006, 152, pp.1395-406. ⟨10.1099/mic.0.28652-0⟩
Microbiology, 2006, 152 (Pt 5), pp.1395-406. ⟨10.1099/mic.0.28652-0⟩
ISSN: 1350-0872
1465-2080
DOI: 10.1099/mic.0.28652-0⟩
Popis: Glycine betaine (GB) and its immediate precursors choline and carnitine, dimethylsulfonioacetate, dimethylsulfoniopropionate, ectoine and proline were effective osmoprotectants for Pseudomonas aeruginosa, but pipecolate, trehalose and sucrose had no osmoprotective effect. GB was accumulated stably or transiently when succinate or glucose, respectively, was used as a carbon and energy source. The catabolite repression mediated by succinate occurred at both low and high salinities, and it did not involve the global regulators Vfr and Crc. A proteomic analysis showed that at least 21 proteins were induced when GB was used as a carbon and energy source, and provided evidence that succinate repressed the synthesis of all these proteins. Many of the proteins induced by GB (sarcosine oxidase, serine hydroxymethyltransferase and serine dehydratase) are involved in GB catabolism. In addition, GB uptake was stimulated at high medium osmolalities but it was insensitive to catabolite repression by succinate. Despite its ability to inhibit betaine catabolism, succinate did not allow any better growth of P. aeruginosa cells under hyperosmotic constraint. Conversely, as observed for cells supplied with glucose, a transient accumulation of GB was sufficient to provide a significant cell osmoprotection.
Databáze: OpenAIRE
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