X-ray analyses of DNA dodecamers containing 2'-deoxy-5-formyluridine
| Autor: | Naoko Karino, Tomoko Sunami, Akio Takénaka, Akira Matsuda, T. Sakaue, Satoko Naito, Masaru Tsunoda, Yoshihito Ueno |
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| Rok vydání: | 2003 |
| Předmět: |
Models
Molecular Speed wobble Base pair DNA polymerase Stereochemistry genetic processes information science DNA-Directed DNA Polymerase Crystallography X-Ray chemistry.chemical_compound heterocyclic compounds Base Pairing biology Transition (genetics) Base Sequence Mutagenesis DNA replication Hydrogen Bonding General Medicine DNA Deoxyuridine Thymine chemistry biological sciences health occupations biology.protein |
| Zdroj: | Nucleic acids research. Supplement (2001). (1) |
| Popis: | It is known that formylation of thymine base induces purine transition in DNA replication. In order to establish the structural basis for such mutagenesis, crystal structures of two kinds of DNA dodecamers d(CGCGRATf5UCGCG) with f5U = 2'-deoxy-5-formyluridine and R = A or G have been determined. The f5U residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being the first example. Structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A. |
| Databáze: | OpenAIRE |
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