The retinal pigment epithelium as a gateway for monocyte trafficking into the eye
Autor: | Kitty Reemst, Inbal Benhar, Michal Schwartz, Vyacheslav Kalchenko |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Green Fluorescent Proteins Central nervous system CX3C Chemokine Receptor 1 Gene Expression Glutamic Acid Vascular Cell Adhesion Molecule-1 Mice Transgenic Retinal Pigment Epithelium Biology Monocytes General Biochemistry Genetics and Molecular Biology Autoimmune Diseases 03 medical and health sciences chemistry.chemical_compound Receptors HIV Leukocyte Trafficking medicine Choroid Plexus Epithelium Animals Receptors Cytokine Molecular Biology Retina Retinal pigment epithelium General Immunology and Microbiology General Neuroscience Monocyte Retinitis Retinal Articles eye diseases Cell biology Mice Inbred C57BL Chemotaxis Leukocyte 030104 developmental biology medicine.anatomical_structure chemistry Optic Nerve Injuries Immunology Optic nerve sense organs |
Zdroj: | The EMBO Journal. 35:1219-1235 |
ISSN: | 1460-2075 0261-4189 |
Popis: | The choroid plexus epithelium within the brain ventricles orchestrates blood‐derived monocyte entry to the central nervous system under injurious conditions, including when the primary injury site is remote from the brain. Here, we hypothesized that the retinal pigment epithelium (RPE) serves a parallel role, as a gateway for monocyte trafficking to the retina following direct or remote injury. We found elevated expression of genes encoding leukocyte trafficking determinants in mouse RPE as a consequence of retinal glutamate intoxication or optic nerve crush (ONC). Blocking VCAM‐1 after ONC interfered with monocyte infiltration into the retina and resulted in a local pro‐inflammatory cytokine bias. Live imaging of the injured eye showed monocyte accumulation first in the RPE, and subsequently in the retina, and peripheral leukocytes formed close contact with the RPE. Our findings further implied that the ocular milieu can confer monocytes a phenotype advantageous for neuroprotection. These results suggest that the eye utilizes a mechanism of crosstalk with the immune system similar to that of the brain, whereby epithelial barriers serve as gateways for leukocyte entry. |
Databáze: | OpenAIRE |
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